Johnson P, Dawson A M, Mould D L
Res Vet Sci. 1983 Sep;35(2):165-70.
Polyacrylamide gel electrophoresis was used to compare serum taken from ponies before and during clinical illness confirmed as grass sickness. A consistent rise in the level of haptoglobin was seen in serum from animals which had shown symptoms for more than two days. Serum albumin was also shown to have altered mobility at the onset of clinical disease. Estimation of the haemoglobin-binding capacity confirmed the haptoglobin increase. This haptoglobin has been purified and some of its properties determined. In contrast to the situation in acute inflammatory conditions no other acute-phase proteins increased to a significant extent in grass sickness. It is concluded that the neurotoxin known to be present in sera of animals experiencing acute grass sickness cannot itself be detected by polyacrylamide gel electrophoresis but may be bound to serum albumin.
采用聚丙烯酰胺凝胶电泳法，比较临床确诊为青草搐搦病的矮种马发病前和发病期间采集的血清。患病超过两天的动物血清中，触珠蛋白水平持续升高。血清白蛋白在临床疾病发作时迁移率也发生了改变。血红蛋白结合能力的测定证实了触珠蛋白的增加。已对该触珠蛋白进行了纯化，并测定了其部分特性。与急性炎症情况不同，在青草搐搦病中，没有其他急性期蛋白显著增加。得出的结论是，已知存在于患急性青草搐搦病动物血清中的神经毒素本身无法通过聚丙烯酰胺凝胶电泳检测到，但可能与血清白蛋白结合。
