Gilmour J S, Mould D L
Res Vet Sci. 1977 Jan;22(1):1-4.
Plasma, serum and cells were prepared from blood taken from acute cases of grass sickness, and plasma was fractionated by gel filtration and salt precipatation. These preparations were all tested for neurotoxic activity by injection into ponies. Plasma and serum were found to produce the neurohistological changes seen in grass sickness, as was a plasma protein fraction of molecular weight 30,000 or greater. Activity was retained following storage at--75 degrees C for 15 months. Plasma given orally to a pony produced no detectable effect, nor was activity demonstrated following the injection of plasma or the high molecular weight fraction to mice, rats, guinea-pigs or rabbits.
从青草搐搦急性病例采集的血液中制备血浆、血清和细胞，血浆通过凝胶过滤和盐沉淀进行分级分离。通过注射到小马体内对这些制剂进行神经毒性活性测试。发现血浆和血清会产生青草搐搦中所见的神经组织学变化，分子量为30000或更大的血浆蛋白级分也是如此。在-75℃储存15个月后活性仍然保留。口服给予小马血浆未产生可检测到的效果，向小鼠、大鼠、豚鼠或兔子注射血浆或高分子量级分后也未显示出活性。
