Soni Rani, Tandon Dhananjay, Hassan Sahina, Samal Debashish, Sharma Divakar
Department of Applied Science, Shri Rawatpura Sarkar University, Raipur 492016, Chhattisgarh, India.
Department of Microbiology, Late Baliram Kashyap Memorial Government Medical College, Jagdalpur 494001, Chhattisgarh, India.
Can J Infect Dis Med Microbiol. 2025 Mar 14;2025:7569212. doi: 10.1155/cjid/7569212. eCollection 2025.
The dengue virus is a significant re-emerging arbovirus drawing global public health concern. Urbanization, population growth, human mobility, water access, and storage practices contribute to its transmission. This hospital-based cross-sectional study is designed to determine dengue infection and prevalence in the district Bastar, Chhattisgarh. Blood samples were collected from the patients, and based on fever duration, they were tested for nonstructural protein 1 (NS1) antigen and immunoglobulin M (IgM) antibody detection. NS1 positive cases were further tested by RT-PCR for serotyping. Among the 2223 collected samples, 2041 were screened for NS1 and 182 for IgM; among them, the positivity was 55 (2.70%) in NS1 and 23 (12.63%) in IgM, respectively. Overall positivity of the dengue cases was 78 (3.51%); however, sex-wise, male and female, dengue positive cases were 45 and 33, respectively. NS1 was positive in 55 cases (70.51%), and IgM in 23 (29.49%) patients. Among these 78 cases, 4 NS1 and 2 IgM cases have shown symptoms of warning signs, while the rest of the cases have shown nonwarning symptoms. Among the 55 NS1 positive cases, the age group (21-60 years) was most affected by 45 (81.81%) DENV cases and the prevalent serotype was DENV-2 in singly and DENV-1 and DENV-2 in combination. The study's serotyping data might signify the early detection and identification of circulating serotypes, which provides valuable insights to clinicians for managing dengue infections. Hence, continuous epidemiological surveillance of DENV in the area is essential to anticipate future heterologous infections and their impact on healthcare. Early detection and vigilant monitoring of patients are crucial for identifying the circulating serotypes of dengue virus, facilitating subsequent epidemiological studies and disease control strategies.
登革病毒是一种重要的再次出现的虫媒病毒，引起了全球公共卫生关注。城市化、人口增长、人口流动、水的获取和储存方式都促成了其传播。这项基于医院的横断面研究旨在确定恰蒂斯加尔邦巴斯塔尔地区的登革热感染情况和患病率。从患者身上采集血样，并根据发热持续时间，对其进行非结构蛋白1（NS1）抗原和免疫球蛋白M（IgM）抗体检测。NS1阳性病例进一步通过逆转录聚合酶链反应（RT-PCR）进行血清分型。在收集的2223份样本中，2041份进行了NS1筛查，182份进行了IgM筛查；其中，NS1阳性率为55例（2.70%），IgM阳性率为23例（12.63%）。登革热病例的总体阳性率为78例（3.51%）；然而，按性别划分，男性和女性的登革热阳性病例分别为45例和33例。NS1在55例（70.51%）病例中呈阳性，IgM在23例（29.49%）患者中呈阳性。在这78例病例中，4例NS1和2例IgM病例出现了警示症状，其余病例出现了非警示症状。在55例NS1阳性病例中，年龄组（21 - 60岁）受登革病毒（DENV）病例影响最大，有45例（81.81%），流行血清型为单一的DENV - 2以及组合的DENV - 1和DENV - 2。该研究的血清分型数据可能意味着对流行血清型的早期检测和识别，这为临床医生管理登革热感染提供了有价值的见解。因此，对该地区登革病毒进行持续的流行病学监测对于预测未来的异源感染及其对医疗保健的影响至关重要。对患者的早期检测和密切监测对于识别登革病毒的流行血清型至关重要，有助于后续的流行病学研究和疾病控制策略。
