Fiolita Shierin V, Aini Rasyid Firda Q, Nurhalifah Lilis, Joelijanto Rudy, Devi Leliana S, Ramadhani Vanda, Harmono Happy, Kusumawardani Banun, Fadiyah Syafika N, Martin Millenieo
Department of Dentistry, Faculty of Dentistry, University of Jember, Jember, Indonesia.
Department of Orthodontics, Faculty of Dentistry, University of Jember, Jember, Indonesia.
J Taibah Univ Med Sci. 2025 Mar 2;20(2):129-138. doi: 10.1016/j.jtumed.2025.02.005. eCollection 2025 Apr.
Malocclusion can be corrected with orthodontic treatment to improve function and aesthetics. Orthodontic treatment patients have the potential to produce greater Reactive Oxygen Species (ROS), as evidenced by an increase in the Oxidative Status Index (OSI) a week after treatment. As a result, oxidative stress may induce an imbalance of bone remodeling through intensifying osteoclastogenesis by expressing pro-inflammatory cytokine, namely Interleukin-6 (IL-6). The worst pathological condition that can occur is Orthodontic-Induced Root Resorption (OIRR), a resorptive area on the root that is unwanted from orthodontic treatment. OIRR incidence is reported to be more than 90 % asymptomatic. Using Forastero cacao bean ( L.) extract gel as a natural antioxidant and anti-inflammatory compound is expected to be able to prevent excess ROS, which triggers an increase in various pro-inflammatory cytokines, especially IL-6, which plays a role in the bone resorption pathway, thus reducing the incidence of OIRR and making orthodontic treatment successful through balanced bone remodeling.
This study aimed to investigate the effect of Forastero cacao bean ( L.) extract gel on the decrease of IL-6 cytokine expressed by osteoblasts, osteoclasts, and osteocytes in the OIRR model of male Wistar rats.
IL-6 positive osteoblasts, osteoclasts, and osteocytes decreased significantly after 8 % Forastero cacao bean extract gel-treated, as seen in T and T groups compared to C and C groups (p < 0.05).
8 % Forastero cacao bean extract gel decreased IL-6-positive osteoblasts, osteoclasts, and osteocytes in the tension side of the alveolar bone.
错牙合畸形可通过正畸治疗得以矫正，从而改善功能和美观。正畸治疗患者有可能产生更多的活性氧（ROS），治疗一周后氧化状态指数（OSI）升高即证明了这一点。因此，氧化应激可能通过表达促炎细胞因子（即白细胞介素-6，IL-6）强化破骨细胞生成，从而导致骨重塑失衡。可能出现的最严重病理状况是正畸诱导的牙根吸收（OIRR），这是正畸治疗中牙根上出现的不良吸收区域。据报道，OIRR的发生率超过90%且无症状。使用福斯托罗可可豆（L.）提取物凝胶作为天然抗氧化剂和抗炎化合物，有望能够预防过量ROS，而过量ROS会引发各种促炎细胞因子增加，尤其是在骨吸收途径中起作用的IL-6，从而降低OIRR的发生率，并通过平衡的骨重塑使正畸治疗取得成功。
本研究旨在探讨福斯托罗可可豆（L.）提取物凝胶对雄性Wistar大鼠OIRR模型中成骨细胞、破骨细胞和骨细胞表达的IL-6细胞因子减少的影响。
与C组和C组相比，在T组和T组中可见，经8%福斯托罗可可豆提取物凝胶处理后，IL-6阳性成骨细胞、破骨细胞和骨细胞显著减少（p < 0.05）。
8%福斯托罗可可豆提取物凝胶可减少牙槽骨张力侧IL-6阳性成骨细胞、破骨细胞和骨细胞。
