Li Tong, Yang Xiao-Hui, Shao Ming-Ju, Dong Yu-Xia, Li Lin-Yu, Lin Cun-Zhi
Department of Respiratory and Critical Care Medicine, The Affiliated Hospital of Qingdao University, Qingdao, 266003, China.
Sci Rep. 2025 Mar 24;15(1):10062. doi: 10.1038/s41598-025-94990-3.
This study aims to investigate the effect and mechanism of photodynamic therapy (PDT) combined with cisplatin on human lung adenocarcinoma A549 cells transplanted tumors in nude mice, and to provide a theoretical basis for clinical PDT. Construction of a nude mouse lung cancer transplantation tumor model using the human lung adenocarcinoma A549 cell line, and the mice were randomly divided into four groups: the control group, the cisplatin alone group, the PDT alone group, and the cisplatin combined PDT group. The apoptosis of tumor cells in the four groups was observed and compared by the TUNEL method, and the mRNA expression levels of apoptosis-related genes Bax, caspase-3 and Survivin, as well as the expression levels of the corresponding proteins, were detected by the real-time fluorescence quantitative polymerase chain reaction (RT-qPCR) and the protein immunoblotting technique (Western blot) respectively. The results showed that photodynamic force combined with cisplatin was effective in inhibiting tumor growth, and its effect was superior to that of cisplatin or PDT alone. This may be related to the promotion of apoptosis, specifically through the up-regulation of Bax and caspase-3, and the down-regulation of Survivin gene expression, thus inhibiting cell proliferation.
本研究旨在探讨光动力疗法（PDT）联合顺铂对人肺腺癌A549细胞裸鼠移植瘤的作用及机制，为临床光动力治疗提供理论依据。利用人肺腺癌A549细胞系构建裸鼠肺癌移植瘤模型，将小鼠随机分为四组：对照组、单纯顺铂组、单纯光动力疗法组和顺铂联合光动力疗法组。采用TUNEL法观察并比较四组肿瘤细胞的凋亡情况，分别用实时荧光定量聚合酶链反应（RT-qPCR）和蛋白质免疫印迹技术（Western blot）检测凋亡相关基因Bax、caspase-3和Survivin的mRNA表达水平以及相应蛋白的表达水平。结果表明，光动力疗法联合顺铂能有效抑制肿瘤生长，其效果优于单纯顺铂或单纯光动力疗法。这可能与促进细胞凋亡有关，具体表现为上调Bax和caspase-3，下调Survivin基因表达，从而抑制细胞增殖。
