Sinclair Patricia, Jeffries William, Lebert Nadege, Saeed Maheen, Ullah Aman, Kabbani Nadine
Interdiscplinary Program in Neuroscience, George Mason University, Fairfax, Virginia, United Sates of America.
Bioinformatics and Computational Biology, George Mason University, Manassas, Virginia, United Sates of America.
PLoS One. 2025 Mar 25;20(3):e0320219. doi: 10.1371/journal.pone.0320219. eCollection 2025.
Neuroinflammation is a key feature of human neurodisease including neuropathy and neurodegenerative disease and is driven by the activation microglia, immune cells of the nervous system. During activation microglia release pro-inflammatory cytokines as well as reactive oxygen species (ROS) that can drive local neuronal and glial damage. Phytocannabinoids are an important class of naturally occurring compounds found in the cannabis plant (Cannabis sativa) that interact with the body's endocannabinoid receptor system. Cannabidiol (CBD) is a prototype phytocannabinoid with anti-inflammatory properties observed in cells and animal models. We measured ROS in human microglia (HMC3) cells using CellROX, a fluorescent dynamic ROS indicator. We tested the effect of CBD on ROS level in the presence of three known immune activators: lipopolysaccharide (LPS), amyloid beta (Aβ42), and human immunodeficiency virus (HIV) glycoprotein (GP120). Confocal microscopy images within microglia were coupled to a deep learning model using a convolutional neural network (CNN) to predict ROS responses. Our study demonstrates a deep learning platform that can be used in the assessment of CBD effect in immune cells using ROS image measure.
神经炎症是包括神经病变和神经退行性疾病在内的人类神经疾病的一个关键特征，它是由小胶质细胞（神经系统的免疫细胞）的激活所驱动的。在激活过程中，小胶质细胞会释放促炎细胞因子以及活性氧（ROS），这些物质会导致局部神经元和神经胶质细胞损伤。植物大麻素是在大麻植物（大麻）中发现的一类重要的天然化合物，它们与人体的内源性大麻素受体系统相互作用。大麻二酚（CBD）是一种原型植物大麻素，在细胞和动物模型中具有抗炎特性。我们使用一种荧光动态ROS指示剂CellROX测量了人小胶质细胞（HMC3）中的ROS。我们在三种已知的免疫激活剂存在的情况下测试了CBD对ROS水平的影响，这三种激活剂分别是脂多糖（LPS）、β-淀粉样蛋白（Aβ42）和人类免疫缺陷病毒（HIV）糖蛋白（GP120）。使用卷积神经网络（CNN）将小胶质细胞内的共聚焦显微镜图像与深度学习模型相结合，以预测ROS反应。我们的研究展示了一个深度学习平台，该平台可用于通过ROS图像测量来评估CBD在免疫细胞中的作用。
