一种仿生双模拟引物对实验性离子释放粘结系统粘结性能的影响——一项体外研究。

Effects of a biomimetic dual-analogue primer on the bonding performance of an experimental ion-releasing adhesive system - An in vitro study.

作者信息

Chou Yu-Fu, Maciel Pires Paula, D'Urso Diego, Ozan Günçe, Mazzitelli Claudia, Maravic Tatjana, Sancaklı Hande Şar, Breschi Lorenzo, Sauro Salvatore

机构信息

Dental Biomaterials and Minimally Invasive Dentistry, Department of Dentistry, Cardenal Herrera-CEU University, CEU Universities, C/Santiago Ramón y Cajal, s/n., Alfara del Patriarca, 46115, Valencia, Spain; Department of Pediatric Dentistry and Orthodontics, Universidade Federal do Rio de Janeiro, Rio de Janeiro, 21941-853, Brazil.

出版信息

J Dent. 2025 May;156:105712. doi: 10.1016/j.jdent.2025.105712. Epub 2025 Mar 23.

DOI:10.1016/j.jdent.2025.105712

PMID:40132789

Abstract

OBJECTIVES

This study evaluated at baseline (T0) and after thermocycling (TC) the dentine bonding performance of an experimental bonding system containing fluoride-doped calcium phosphate (FDCP) used in combination with a biomimetic dual-analogue dentine conditioner (PRM). Their effect on dentine enzymatic activity was also evaluated, along with fractographic and ultramorphology/nanoleakage characteristics.

METHODS

An experimental FDCP-containing primer and bond resin system was generated and applied on dentine with or without PRM. Controls included a commercial universal adhesive (SCH) and a glass ionomer cement (GIC). Resin-dentine matchsticks were created and subjected to microtensile bond strength (µTBS) testing at 24 h or after thermo-aging (10,000 cycles). Failure mode and fractographic SEM analyses were performed post-µTBS test. Interfacial characteristics and in situ zymography were evaluated through confocal microscopy at T0 and after TC. Gelatine zymography was performed on protein extracts from dentine powder pretreated with the tested materials. Data from the µTBS test were statistically analysed (α = 0.05).

RESULTS

SCH showed a significant drop in µTBS (p < 0.05) with adhesive failures and collagen degradation often observed after TC. GIC specimens had the lowest µTBS values (p < 0.05), but no significant drop after TC (p > 0.05). The experimental system was characterised by lower µTBS values than SCH, but with no significant drop after TC (p > 0.05). However, specimens in the experimental groups showed at the bonding interface evident mineral deposition, with less enzymatic activity compared to controls.

CONCLUSIONS

The FDCP-containing experimental adhesive system maintained the bonding performance after TC, protecting the hybrid layer especially when used in combination with PRM.

CLINICAL SIGNIFICANCE

Innovative ion-releasing restorative systems play a crucial role in preserving the integrity of hybrid layers. These systems promote a remineralisation process that not only reinforces the hybrid layers but also inhibits dentine proteolytic enzymes, thereby enhancing the chances of creating long-lasting dental restorations.

摘要

目的

本研究在基线期（T0）和热循环（TC）后，评估了一种含氟掺杂磷酸钙（FDCP）的实验性粘结系统与仿生双模拟牙本质调节剂（PRM）联合使用时的牙本质粘结性能。还评估了它们对牙本质酶活性的影响，以及断口形貌和超微形态/纳米渗漏特征。

方法

制备了一种含FDCP的实验性底漆和粘结树脂系统，并将其应用于有或无PRM的牙本质上。对照组包括一种市售通用粘结剂（SCH）和一种玻璃离子水门汀（GIC）。制作树脂-牙本质火柴棒，并在24小时或热老化（10000次循环）后进行微拉伸粘结强度（µTBS）测试。在µTBS测试后进行失效模式和断口形貌扫描电子显微镜分析。通过共聚焦显微镜在T0和TC后评估界面特征和原位酶谱分析。对用测试材料预处理的牙本质粉末的蛋白质提取物进行明胶酶谱分析。对µTBS测试数据进行统计学分析（α = 0.05）。

结果

SCH的µTBS显著下降（p < 0.05），热循环后常观察到粘结失败和胶原降解。GIC标本的µTBS值最低（p < 0.05），但热循环后无显著下降（p > 0.05）。实验系统的特点是µTBS值低于SCH，但热循环后无显著下降（p > 0.05）。然而，实验组的标本在粘结界面显示出明显的矿物质沉积，与对照组相比酶活性较低。