Chou Yu Fu, Maciel Pires Paula, Alambiaga-Caravaca Adrian M, Spagnuolo Gianrico, Hibbitts Alan, Sauro Salvatore
Dental Biomaterials and Minimally Invasive Dentistry, Department of Dentistry, Cardenal Herrera-CEU University, CEU Universities, Valencia, Spain.
Department of Pediatric Dentistry, Universidade Federal Do Rio de Janeiro, Rio de Janeiro, Brazil.
J Dent. 2025 Jan;152:105468. doi: 10.1016/j.jdent.2024.105468. Epub 2024 Nov 17.
Evaluate the remineralisation ability of experimental resin-based materials containing Fluoride-Doped Calcium Phosphate (FDCP), applied in mineral-deficient dentine in combination with a biomimetic dual-analogue primer.
Artificial dentine carious lesions were created in occlusal cavities of human molars. An experimental resin-based adhesive and flowable composite, containing FDCP (10wt% and 20wt%, respectively), were applied (±) with a biomimetic primer containing polyacrylic acid and sodium tripolyphosphate. A glass-ionomer cement and a conventional adhesive/composite were used as comparative materials. All specimens were submitted to chewing simulation in artificial saliva (AS). Subsequently, these were cut into slabs and submitted to Knoop microhardness testing and Raman spectroscopy (AS: 0, 15, 30 and 60 days). Further specimens were analysed through confocal scanning microscopy (AS: 0, 60 days). Total demineralised dentine and disc-shape materials were analysed through FTIR, SEM and EDX (Ca/P ratio) after prolonged contact in AS. Data were statistically analysed (two-way ANOVA and multiple comparison Bonferroni; α=0.05).
Only the experimental FDCP materials induced a significant increase in microhardness after storage in AS, especially in those specimens pre-treated with the biomimetic primer, except at 50 µm underneath the interface. Raman and CLSM analysis corroborated such results. The ability of the experimental FDCP resin-based materials to induce apatite-like deposition was demonstrated through SEM-EDX and FTIR.
Resin-based materials containing FDCP can mineralise the mineral-deficient dentine, especially when combined with a biomimetic dual-analogue primer.
Such Innovative ion-releasing restorative systems applied after selective caries removal might promote remineralisation of fully and partially demineralised dentine and increase the durability of dental restorations.
评估含氟掺杂磷酸钙（FDCP）的实验性树脂基材料与仿生双模拟底漆联合应用于矿物质缺乏牙本质时的再矿化能力。
在人磨牙的咬合面窝洞中制备人工牙本质龋损。应用含FDCP（分别为10wt%和20wt%）的实验性树脂基粘合剂和流动复合树脂，（±）联合含聚丙烯酸和三聚磷酸钠的仿生底漆。使用玻璃离子水门汀和传统粘合剂/复合树脂作为对照材料。所有标本在人工唾液（AS）中进行咀嚼模拟。随后，将这些标本切成薄片，并进行努氏显微硬度测试和拉曼光谱分析（AS：0、15、30和60天）。通过共聚焦扫描显微镜对更多标本进行分析（AS：0、60天）。在AS中长时间接触后，通过傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）和能谱分析（Ca/P比）对完全脱矿的牙本质和盘状材料进行分析。对数据进行统计学分析（双向方差分析和多重比较Bonferroni检验；α=0.05）。
仅实验性FDCP材料在AS中储存后诱导显微硬度显著增加，特别是在用仿生底漆预处理的标本中，界面下方50 µm处除外。拉曼光谱和共聚焦激光扫描显微镜分析证实了这些结果。通过SEM-EDX和FTIR证明了实验性FDCP树脂基材料诱导类磷灰石沉积的能力。
含FDCP的树脂基材料可使矿物质缺乏的牙本质矿化，尤其是与仿生双模拟底漆联合使用时。
在选择性龋病去除后应用这种创新的离子释放修复系统可能促进完全和部分脱矿牙本质的再矿化，并提高牙科修复体的耐久性。
