Jaidane Nadia, Tilouche Lamia, Oueslati Saoussen, Girlich Delphine, Azaiez Sana, Jacquemin Aymeric, Dortet Laurent, Naija Walid, Trabelsi Abdelhalim, Naas Thierry, Mansour Wejdene, Bonnin Rémy A
Laboratory of Metabolic Biophysics and Applied Pharmacology (LR12ES02), Department of Biophysics, Faculty of Medicine Ibn El Jazzar of Sousse, University of Sousse, Sousse 4000, Tunisia.
Clinical Microbiology Laboratory, University Hospital of Sahloul, Sousse 4002, Tunisia.
Pathogens. 2025 Mar 20;14(3):298. doi: 10.3390/pathogens14030298.
() is an opportunistic pathogen involved in urinary tract infections as well as various nosocomial infections. Emerging resistances to beta-lactams in this species complicates potential treatment since it is intrinsically resistant to colistin. Eleven isolates of carbapenem-non-susceptible were identified in Sousse Hospital, Tunisia, from January 2018 to December 2022. MICs were determined and isolates were sequenced to determine their resistomes, sequence types, virulence factors, and their clonal relationships. Susceptibility testing showed that all isolates were resistant to carbapenems, aminoglycosides, fluoroquinolones, chloramphenicol, and the trimethoprim/sulfamethoxazole combination. They remained susceptible to the aztreonam/avibactam combination. All isolates produced NDM-1 carbapenemase and ArmA 16S rRNA methylase. In addition, one isolate co-produced the gene. All isolates belonged to ST135, and phylogenetic analysis revealed that they were closely related. This study described the first outbreak of NDM-1-producing in Tunisia.
（某菌）是一种机会致病菌，可引发尿路感染及多种医院感染。该菌种对β-内酰胺类药物出现的新耐药性使潜在治疗变得复杂，因为它对黏菌素具有固有耐药性。2018年1月至2022年12月期间，在突尼斯苏塞医院鉴定出11株对碳青霉烯类不敏感的（某菌）分离株。测定了最低抑菌浓度（MIC），并对分离株进行测序以确定其耐药组、序列类型、毒力因子及其克隆关系。药敏试验表明，所有分离株均对碳青霉烯类、氨基糖苷类、氟喹诺酮类、氯霉素以及甲氧苄啶/磺胺甲恶唑合剂耐药。它们对氨曲南/阿维巴坦合剂仍敏感。所有分离株均产生NDM-1碳青霉烯酶和ArmA 16S rRNA甲基化酶。此外，一株分离株还共同产生了（另一）基因。所有分离株均属于ST135，系统发育分析表明它们密切相关。本研究描述了突尼斯首次出现的产NDM-1（某菌）疫情。
