Eintrei C, Leszniewski W, Odman S, Lewis D H
Acta Physiol Scand. 1985 Jun;124(2):261-7. doi: 10.1111/j.1748-1716.1985.tb07660.x.
A method for measuring regional cerebral blood flow has been developed. On pig brain cortex a 1 cm2 size polyester film was placed, under which 0.6-1.3 mCi of 133Xenon in 2-4 microliter of saline was applied atraumatically. The wash-out process was registered with an external detector, and can be described as a sum of monoexponential functions. The first component of the curve, obtained by curve resolution, indicates blood flow in grey matter and the second slow component indicates blood flow in white matter. When total ischaemia was induced, there was no wash-out of the isotope. Freezing the brain after isotope application at different stages during the wash-out showed isotope in both grey and white matter. The isotope did not diffuse into the polyester film. This technique was also used in studies on the spinal medulla where white matter is outermost and grey innermost. The wash-out curve obtained consisted of only one monoexponential function; blood flow from grey matter was not present in the wash-out curve. For calculation of cerebral blood flow a modified two-compartment model was used. It is concluded that this method measures local cerebral blood flow in both grey and white matter. The method can be used clinically to measure the local cerebral blood flow during neurosurgical operations.
一种测量局部脑血流量的方法已经开发出来。在猪脑皮层上放置一块1平方厘米大小的聚酯薄膜，在其下方无创地施加2至4微升盐水中含有0.6 - 1.3毫居里的133氙。用外部探测器记录洗脱过程，该过程可用单指数函数之和来描述。通过曲线解析得到的曲线的第一个成分表示灰质中的血流量，第二个缓慢成分表示白质中的血流量。当诱导完全缺血时，没有同位素的洗脱。在洗脱过程中的不同阶段施加同位素后冷冻大脑，结果显示灰质和白质中均有同位素。同位素没有扩散到聚酯薄膜中。该技术也用于脊髓研究，脊髓中白质在外层，灰质在最内层。得到的洗脱曲线仅由一个单指数函数组成；洗脱曲线中没有灰质的血流量。为了计算脑血流量，使用了一种改进的双室模型。得出的结论是，该方法可测量灰质和白质中的局部脑血流量。该方法可在临床上用于神经外科手术期间测量局部脑血流量。
