Chen Zhou, Song Yanyin, Yan Yuhan, Chen Wei, Ren Tong, Ma Aijin, Li Siting, Jia Yingmin
Beijing Technology and Business University, Beijing 100048, China.
Beijing Technology and Business University, Beijing 100048, China.
Food Chem. 2025 Jul 15;480:143948. doi: 10.1016/j.foodchem.2025.143948. Epub 2025 Mar 19.
A novel cellobiose 2-epimerase gene (CsCEase1) from Clostridium sp. TW13 was successfully overexpressed. CsCEase1, with a molecular weight of 45.0 kDa, exhibited a specific activity of 254.75 U/mg and showed optimal activity at 40 °C and pH 7.5. Using CsCEase1, 44.30 % of lactose was converted into epilactose, yielding a production level of 26.58 g/L. An efficient purification method was developed, incorporating crystallization and β-galactosidase treatment to remove the unconverted lactose, while Saccharomyces cerevisiae was used to consume the produced glucose. Final purification with activated carbon resulted in epilactose with 98 % purity and a recovery rate of 66.47 %. Furthermore, CsCEase1 was incubated with whey power and expired milk, achieving epilactose conversion rates of 29.65 % and 32.69 %, respectively. These findings highlight the potential of CsCEase1 for the cost-effective and environmentally sustainable production of epilactose from dairy waste.
来自梭菌属TW13的一种新型纤维二糖2-表异构酶基因（CsCEase1）成功实现了过表达。CsCEase1的分子量为45.0 kDa，比活性为254.75 U/mg，在40°C和pH 7.5时表现出最佳活性。使用CsCEase1，44.30%的乳糖被转化为表乳糖，产量水平为26.58 g/L。开发了一种高效的纯化方法，包括结晶和β-半乳糖苷酶处理以去除未转化的乳糖，同时使用酿酒酵母消耗产生的葡萄糖。用活性炭进行最终纯化得到纯度为98%的表乳糖，回收率为66.47%。此外，将CsCEase1与乳清粉和过期牛奶一起孵育，表乳糖转化率分别达到29.65%和32.69%。这些发现突出了CsCEase1从乳制品废料中经济高效且环境可持续地生产表乳糖的潜力。
