内寄生蜂微红盘绒茧蜂的染色体水平基因组组装

Chromosome-level genome assembly of an endoparasitoid Cotesia ruficrus.

作者信息

Zhao Xianxin, Liu Yuanyuan, Ye Xinhai, Wang Fang, Fang Qi, Ye Gongyin

机构信息

State Key Laboratory of Rice Biology and Breeding & Ministry of Agricultural and Rural Affairs Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Institute of Insect Sciences, Zhejiang University, Hangzhou, China.

College of Advanced Agriculture Sciences, Zhejiang A&F University, Hangzhou, China.

出版信息

Sci Data. 2025 Mar 26;12(1):508. doi: 10.1038/s41597-025-04837-9.

DOI:10.1038/s41597-025-04837-9

PMID:40140407

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11947152/

Abstract

Cotesia ruficrus is a gregarious larval endoparasitoid of many important agricultural pest moths, such as the fall armyworm, northern armyworm and rice leaffolder. While eight of Cotesia genomes had been reported, high-quality genomic data of C. ruficrus has yet to be performed. This study presents a chromosome-scale genome assembly of C. ruficrus, utilizing both PacBio HiFi-reads and Hi-C reads. The genome size is about 185.3 Mb harboring 10 chromosomes, and 209 unanchored scaffolds with a scaffold N50 length of 15.81 Mb. The anchored rate of sequences to chromosomes is 95.55%. A total of 14,001 genes have been annotated. This work delivers a first high-quality, chromosome-scale genome for C. ruficrus, enriching the datasets for comparative evolutionary studies within the Cotesia genus. Additionally, it offers a valuable genomic resource for future biological and genetic research, as well as for enhancing pest management strategies, such as biological control.

摘要

微红盘绒茧蜂是许多重要农业害虫蛾类的群居幼虫内寄生蜂，如草地贪夜蛾、黏虫和稻纵卷叶螟。虽然已经报道了8个盘绒茧蜂属的基因组，但微红盘绒茧蜂的高质量基因组数据尚未开展。本研究利用PacBio HiFi-reads和Hi-C reads，展示了微红盘绒茧蜂的染色体水平基因组组装。基因组大小约为185.3 Mb，包含10条染色体和209个未锚定的支架，支架N50长度为15.81 Mb。序列在染色体上的锚定率为95.55%。总共注释了14,001个基因。这项工作首次为微红盘绒茧蜂提供了高质量的染色体水平基因组，丰富了盘绒茧蜂属内比较进化研究的数据集。此外，它为未来的生物学和遗传学研究以及加强害虫管理策略（如生物防治）提供了宝贵的基因组资源。