Faraone Immacolata, Russo Daniela, Chiummiento Lucia, Fernandez Eloy, Choudhary Alka, Monné Magnus, Milella Luigi, Rai Dilip K
Department of Science, University of Basilicata, V.le dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
Spinoff BioActiPlant s.r.l., Università della Basilicata, V.le dell'Ateneo Lucano, 10, 85100 Potenza, Italy.
Foods. 2020 Feb 1;9(2):144. doi: 10.3390/foods9020144.
The genus belonging to the Lamiaceae family, and is an important South American mint genus used commonly in folk medicine as an aroma in cooking. The phytochemical-rich samples of the aerial parts of Epling. were tested for pharmacological and health-promoting bioactivities using in vitro chemical and enzymatic assays. A range of radical scavenging activities of the samples against biological radicals such as nitric oxide and superoxide anion and against synthetic 2,2-diphenyl-1-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radicals, the ferric reducing antioxidant power and the lipid peroxidation inhibition were determined and ranked using the 'relative antioxidant capacity index' (RACI). The ethyl acetate fraction showed the highest RACI of +1.12. Analysis of the various fractions' inhibitory ability against enzymes involved in diabetes (α-amylase and α-glucosidase), and against enzymes associated with Parkinson's or Alzheimer's diseases (acetylcholinesterase and butyrylcholinesterase) also suggested that the ethyl acetate fraction was the most active. Liquid chromatography-tandem mass spectrometry analysis of the ethyl acetate fraction showed more than 30 polyphenolic compounds, including triterpenes. The inhibitory cholinesterase effects of the triterpenes identified from were further analysed by in silico docking of these compounds into 3D-structures of acetylcholinesterase and butyrylcholinesterase. This is the first study on pharmacological activities and phytochemical profiling of the aerial parts of , showing that this plant, normally used as food in South America, is also rich in health-promoting phytochemicals.
该属属于唇形科，是一种重要的南美薄荷属植物，在民间医学中常用作烹饪香料。对Epling.地上部分富含植物化学成分的样本进行了体外化学和酶学分析，以测试其药理和促进健康的生物活性。测定了样本对生物自由基如一氧化氮和超氧阴离子以及对合成的2,2-二苯基-1-苦基肼和2,2'-偶氮双(3-乙基苯并噻唑啉-6-磺酸)自由基的一系列自由基清除活性、铁还原抗氧化能力和脂质过氧化抑制能力，并使用“相对抗氧化能力指数”(RACI)进行排名。乙酸乙酯馏分的RACI最高，为+1.12。对各馏分对糖尿病相关酶(α-淀粉酶和α-葡萄糖苷酶)以及与帕金森病或阿尔茨海默病相关酶(乙酰胆碱酯酶和丁酰胆碱酯酶)的抑制能力分析也表明，乙酸乙酯馏分活性最强。对乙酸乙酯馏分进行液相色谱-串联质谱分析，发现了30多种多酚类化合物，包括三萜类化合物。通过将这些化合物与乙酰胆碱酯酶和丁酰胆碱酯酶的三维结构进行计算机对接，进一步分析了从该植物中鉴定出的三萜类化合物的胆碱酯酶抑制作用。这是首次对该植物地上部分的药理活性和植物化学特征进行研究，表明这种在南美通常用作食物的植物也富含促进健康的植物化学物质。
