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用于新型质量一致性评估的DNA标记物筛选及应用

Screening and Application of DNA Markers for Novel Quality Consistency Evaluation in .

作者信息

Cai Siyuan, Liao Xuejiao, Xi Yidan, Chu Yang, Liu Shuang, Su Hang, Dou Deqiang, Xu Jiang, Xiao Shuiming

机构信息

State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-Di Herbs, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.

College of Pharmacy, Liaoning University of Traditional Chinese Medicine, Dalian 116600, China.

出版信息

Int J Mol Sci. 2025 Mar 17;26(6):2701. doi: 10.3390/ijms26062701.

DOI:10.3390/ijms26062701
PMID:40141343
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11942579/
Abstract

Quality control remains a challenge in traditional Chinese medicine (TCM). This study introduced a novel genetic-based quality control method for TCM. Genetic variations in ginseng were evaluated across whole-genome, chloroplast genome, and ITS2 DNA barcode dimensions. Significant genetic variations were found in whole-genome comparison, leading to the use of inter-simple sequence repeat markers to assess the genetic diversity of ginseng decoction pieces (PG), garden ginseng (GG), and ginseng under forest (FG). Fingerprints of ginseng samples revealed instability within some batches. These evaluations were transformed into information entropy to calculate the size of Hardy-Weinberg equilibrium population (HWEP). FG had significantly higher genetic and chemical minimum HWEP than GG ( < 0.05). Notably, a significant positive correlation was observed between the minimum HWEP for genetics and for chemistry ( = 0.857, = 0.014). Genetic polymorphism analysis of ginseng has the potential to evaluate chemical quality consistency, offering a new method to ensure quality consistency in TCM.

摘要

质量控制在中国传统医学（中医）中仍然是一项挑战。本研究引入了一种基于基因的新型中医质量控制方法。在全基因组、叶绿体基因组和ITS2 DNA条形码维度上评估了人参的基因变异。在全基因组比较中发现了显著的基因变异，从而使用简单序列重复区间标记来评估人参饮片（PG）、园参（GG）和林下参（FG）的遗传多样性。人参样品的指纹图谱显示部分批次存在不稳定性。这些评估被转化为信息熵，以计算哈迪-温伯格平衡群体（HWEP）的大小。FG的遗传和化学最小HWEP显著高于GG（<0.05）。值得注意的是，遗传和化学的最小HWEP之间存在显著正相关（=0.857，=0.014）。人参的基因多态性分析有潜力评估化学质量一致性，为确保中医质量一致性提供了一种新方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/681cb0eb6078/ijms-26-02701-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/f57d19ddf13b/ijms-26-02701-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/c61b95d90308/ijms-26-02701-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/891ceacd2296/ijms-26-02701-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/0811f375c2b9/ijms-26-02701-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/681cb0eb6078/ijms-26-02701-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/f57d19ddf13b/ijms-26-02701-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/c61b95d90308/ijms-26-02701-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/891ceacd2296/ijms-26-02701-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/0811f375c2b9/ijms-26-02701-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/57d2/11942579/681cb0eb6078/ijms-26-02701-g005.jpg

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