Wu Cuiling, Lu Qingwei, Ma Shengchao, Mamat Nuramina, Tang Sen, Liu Wenna, Wang Yaqian, Anwar Asma, Lu Yingjie, Ma Qiangqiang, Aimaier Gulinigaer, Fu Xuefeng
Xinjiang Key Laboratory of Special Species Conservation and Regulatory Biology, International Center for the Collaborative Management of Cross-Border Pest in Central Asia, College of Life Science, Xinjiang Normal University, Urumqi 830054, China.
Xinjiang Key Laboratory of Animal Biotechnology, Key Laboratory of Herbivorous Animal Genetics, Breeding and Reproduction, Ministry of Agriculture and Rural Affairs, Institute of Biotechnology, Xinjiang Academy of Animal Sciences, Urumqi 830011, China.
Int J Mol Sci. 2025 Mar 18;26(6):2710. doi: 10.3390/ijms26062710.
Based on comprehensive proteomic analysis conducted across various stages of secondary hair follicles (SHFs), the growth and development regulatory mechanisms of SHFs in Jiangnan cashmere goats were studied. Proteomic analysis of skin tissue from the SHF anagen (An), catagen (Cn), and telogen (Tn) revealed 145 differentially expressed proteins (DEPs) between the An and Tn, 53 DEPs between the Cn and An, and 168 DEPs between the Cn and Tn. Gene Ontology (GO) annotations indicated that the DEPs were predominantly involved in keratin filament formation (KRTAP3-1, KRT1, KRT8), intermediate filament formation (KRT26, KRT35, KRT19, etc.), and lipid metabolism (FA2H, CERS6, ECH1, TECR, etc.). Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis identified significant enrichment of DEPs in pathways related to hair follicle growth and development. Notably, these included the PPAR signaling pathway (PLIN2, PLIN4, ACSL5, etc.), the IL-17 signaling pathway (S100A7A, LOC108633164), and the estrogen signaling pathway (KRT26, KRT35, LOC102176457.). Western blotting (WB) experiments were then performed on five DEPs (KRT28, FA2H, PLIN2, FABP7, and VNN1) to validate the consistency of the WB results with the proteomic data. Overexpression and siRNA interference of in dermal papilla cells (DPCs) were followed by CCK8 and flow cytometry assays, revealing that knockdown significantly decreased DPC proliferation while inducing apoptosis, compared to controls. These findings suggest that the gene plays a crucial role in modulating SHF growth cycles in cashmere goats by influencing DPC proliferation. These results provide novel insights that could inform the development of breeding strategies aimed at enhancing the cashmere yield in such goats.
基于对次生毛囊（SHFs）不同阶段进行的全面蛋白质组学分析，研究了江南绒山羊SHFs的生长发育调控机制。对SHF生长期（An）、退行期（Cn）和休止期（Tn）皮肤组织的蛋白质组学分析显示，An和Tn之间有145种差异表达蛋白（DEPs），Cn和An之间有53种DEPs，Cn和Tn之间有168种DEPs。基因本体论（GO）注释表明，这些DEPs主要参与角蛋白丝形成（KRTAP3-1、KRT1、KRT8）、中间丝形成（KRT26、KRT35、KRT19等）和脂质代谢（FA2H、CERS6、ECH1、TECR等）。此外，京都基因与基因组百科全书（KEGG）通路富集分析确定了DEPs在与毛囊生长发育相关的通路中显著富集。值得注意的是，这些通路包括PPAR信号通路（PLIN2、PLIN4、ACSL5等）、IL-17信号通路（S100A7A、LOC108633164）和雌激素信号通路（KRT26、KRT35、LOC102176457）。然后对5种DEPs（KRT28、FA2H、PLIN2、FABP7和VNN1）进行蛋白质免疫印迹（WB）实验，以验证WB结果与蛋白质组学数据的一致性。在对真皮乳头细胞（DPCs）进行基因的过表达和小干扰RNA（siRNA）干扰后，进行CCK8和流式细胞术检测，结果显示，与对照组相比，基因敲低显著降低了DPC增殖，同时诱导了细胞凋亡。这些发现表明，该基因通过影响DPC增殖，在调节绒山羊SHF生长周期中起关键作用。这些结果提供了新的见解，可为旨在提高此类山羊羊绒产量的育种策略的制定提供参考。
