Faculty of Pharmacy, Isra University, Amman, Jordan.
Department of Chemistry, Faculty of Science, Isra University, Amman, Jordan.
IET Nanobiotechnol. 2022 May;16(3):92-101. doi: 10.1049/nbt2.12081. Epub 2022 Mar 25.
Silver nanoparticles (AgNPs) have shown potential applications in drug delivery. In this study, the AgNPs was prepared from silver nitrate in the presence of alginate as a capping agent. The ciprofloxacin (Cipro) was loaded on the surface of AgNPs to produce Cipro-AgNPs nanocomposite. The characteristics of the Cipro-AgNPs nanocomposite were studied by X-ray diffraction (XRD), UV-Vis, transmission electron microscopy (TEM), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), Fourier-transform infra-red analysis (FT-IR) and zeta potential analyses. The XRD of AgNPs and Cipro-AgNPs nanocomposite data showed that both have a crystalline structure in nature. The FT-IR data indicate that the AgNPs have been wrapped by the alginate and loaded with the Cipro drug. The TEM image showed that the Cipro-AgNPs nanocomposites have an average size of 96 nm with a spherical shape. The SEM image for AgNPs and Cipro-AgNPs nanocomposites confirmed the needle-lumpy shape. The zeta potential for Cipro-AgNPs nanocomposites exhibited a positive charge with a value of 6.5 mV. The TGA for Cipro-AgNPs nanocomposites showed loss of 79.7% in total mass compared to 57.6% for AgNPs which is due to the Cipro loaded in the AgNPs. The release of Cipro from Cipro-AgNPs nanocomposites showed slow release properties which reached 98% release within 750 min, and followed the Hixson-Crowell kinetic model. In addition, the toxicity of AgNPs and Cipro-AgNPs nanocomposites was evaluated using normal (3T3) cell line. The present work suggests that Cipro-AgNPs are suitable for drug delivery.
银纳米粒子（AgNPs）在药物传递方面显示出了潜在的应用。在本研究中，AgNPs 是由硝酸银在海藻酸钠作为封端剂的存在下制备的。环丙沙星（Cipro）被负载在 AgNPs 的表面上，以产生 Cipro-AgNPs 纳米复合材料。通过 X 射线衍射（XRD）、紫外可见光谱（UV-Vis）、透射电子显微镜（TEM）、热重分析（TGA）、扫描电子显微镜（SEM）、傅里叶变换红外分析（FT-IR）和zeta 电位分析研究了 Cipro-AgNPs 纳米复合材料的特性。AgNPs 和 Cipro-AgNPs 纳米复合材料的 XRD 数据表明，它们都具有天然的结晶结构。FT-IR 数据表明，AgNPs 已被海藻酸钠包裹并负载了 Cipro 药物。TEM 图像显示，Cipro-AgNPs 纳米复合材料的平均粒径为 96nm，呈球形。AgNPs 和 Cipro-AgNPs 纳米复合材料的 SEM 图像证实了针状块状的形状。Cipro-AgNPs 纳米复合材料的 zeta 电位呈现出正电荷，值为 6.5mV。Cipro-AgNPs 纳米复合材料的 TGA 显示，与 AgNPs 的 57.6%相比，总质量损失了 79.7%，这是由于 Cipro 负载在 AgNPs 上。Cipro 从 Cipro-AgNPs 纳米复合材料中的释放显示出缓慢释放的特性，在 750 分钟内达到 98%的释放，并遵循 Hixson-Crowell 动力学模型。此外，还使用正常（3T3）细胞系评估了 AgNPs 和 Cipro-AgNPs 纳米复合材料的毒性。本研究表明，Cipro-AgNPs 适合药物传递。
