Liu Lulu, Ge Chuang, Zhang Yu, Ma Wenrui, Su Xi, Chen Li, Li Shunbo, Wang Li, Mu Xiaojing, Xu Yi
School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400044, China..
Biomater Sci. 2020 Sep 7;8(17):4852-4860. doi: 10.1039/d0bm00648c. Epub 2020 Jul 31.
The formation of bacterial biofilms is a key factor in the emergence of chronic infections due to the strong resistance of biofilms to conventional antibiotics. There is an urgent need to develop an effective strategy to control the formation of biofilms. In this study, a nanocomposite of tannic acid and silver (Tannin-AgNPs) was designed and successfully prepared based on the quorum sensing (QS) inhibitory activity of tannic acid and the anti-bacterial activity of silver. The dynamic light scattering and SEM observations indicated that the obtained Tannin-AgNPs were spherical with a mean particle size of 42.37 nm. Tannic acid was successfully modified on the surface of silver nanoparticles and characterized via Fourier transform infrared (FTIR) spectroscopy. The prepared Tannin-AgNPs demonstrated a more effective anti-bacterial and anti-biofilm activity against E. coli than the unmodified AgNPs or tannic acid. In addition, the Tannin-AgNPs can modulate the formation process of E. coli biofilms, shorten the growth period of biofilms and extend the dispersion period of biofilms. Tannin-AgNPs also showed the function of decreasing the production of the QS signal molecule. The proposed strategy of constructing a nanocomposite using AgNPs and natural components with QS inhibitory activity is effective and promising for inhibiting the formation of biofilms.
由于生物膜对传统抗生素具有强大的抗性，细菌生物膜的形成是慢性感染出现的关键因素。迫切需要制定一种有效的策略来控制生物膜的形成。在本研究中，基于单宁酸的群体感应（QS）抑制活性和银的抗菌活性，设计并成功制备了单宁酸与银的纳米复合材料（单宁 - 银纳米颗粒）。动态光散射和扫描电子显微镜观察表明，所获得的单宁 - 银纳米颗粒呈球形，平均粒径为42.37nm。单宁酸成功修饰在银纳米颗粒表面，并通过傅里叶变换红外（FTIR）光谱进行表征。制备的单宁 - 银纳米颗粒对大肠杆菌表现出比未修饰的银纳米颗粒或单宁酸更有效的抗菌和抗生物膜活性。此外，单宁 - 银纳米颗粒可以调节大肠杆菌生物膜的形成过程，缩短生物膜的生长周期并延长生物膜的分散期。单宁 - 银纳米颗粒还显示出降低QS信号分子产生的功能。利用银纳米颗粒和具有QS抑制活性的天然成分构建纳米复合材料的策略对于抑制生物膜的形成是有效且有前景的。
