Krishnan Sukumaran Vipin, Anaswara Prakasan A, Nampoothiri Kesavan Madhavan, Kovács Szilvia, Adácsi Cintia, Miklós Ida, Király Szabina, Pócsi István, Pusztahelyi Tünde
Microbial Processes and Technology Division (MPTD), CSIR-National Institute for Interdisciplinary Science and Technology (NIIST), Thiruvananthapuram 695019, India.
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India.
Microorganisms. 2025 Mar 15;13(3):666. doi: 10.3390/microorganisms13030666.
The biotechnological potential of the lactic acid bacterial genus has not been fully unearthed. Since have not been tested against Fusaria and their mycotoxins, newly isolated strains were characterized and tested for their antifungal capacities on plant pathogens. BF2 and ML2 successfully inhibited NCIM 1100, NCIM 1099, MTCC 2089, and MTCC 284 in co-cultures. Ethyl acetate extracts of the cell-free culture supernatants (CFCS) of also exhibited antifungal activity against the tested fungal cultures. The main mycotoxins of Fusaria were tested against the isolates. In MRS broth, BF2 was resistant to the mycotoxins (deoxynivalenol, zearalenone, T2, and fumonisin B1), while the ML2 strain showed 22.1-24.5% growth inhibition. Meanwhile, neither bacterium showed potential in mycotoxin reduction. The study highlighted that BF2 and ML2 and their CFCS are suitable for growth inhibition, as shown on surface-sterilized peanuts and wheat grains, but not for mycotoxin elimination.
乳酸菌属的生物技术潜力尚未得到充分发掘。由于尚未针对镰刀菌及其霉菌毒素进行测试，因此对新分离的菌株进行了表征，并测试了它们对植物病原体的抗真菌能力。在共培养中，BF2和ML2成功抑制了NCIM 1100、NCIM 1099、MTCC 2089和MTCC 284。这些菌株的无细胞培养上清液（CFCS）的乙酸乙酯提取物也对测试的真菌培养物表现出抗真菌活性。针对分离出的菌株测试了镰刀菌的主要霉菌毒素。在MRS肉汤中，BF2对这些霉菌毒素（脱氧雪腐镰刀菌烯醇、玉米赤霉烯酮、T2和伏马菌素B1）具有抗性，而ML2菌株显示出22.1-24.5%的生长抑制。同时，两种细菌在降低霉菌毒素方面均未显示出潜力。该研究强调，BF2和ML2及其CFCS适用于抑制生长，如在表面消毒的花生和小麦籽粒上所示，但不适用于消除霉菌毒素。
