Gehlert Sebastian, Khurshudyan Asatur, Weber Sebastian, Widdershoven Jochem, Van Schuylenbergh Reinout
Institut für Sportwissenschaft, University of Hildesheim, Hildesheim, Lower Saxony, Germany.
INSCYD GmbH, Salenstein, Switzerland.
Front Sports Act Living. 2025 Mar 12;7:1546117. doi: 10.3389/fspor.2025.1546117. eCollection 2025.
Traditionally, buffer capacity () is measured on muscle biopsies by measuring changes in muscle pH in relation to exposure of standardized quantities of hydrochloric acid. This is an invasive approach requiring specific equipment and trained personnel which limits its usability in a normal training context. Assessing using capillary blood lactate concentration (BLC) and pH values has been proposed as a more practical and cost-effective approach. The reliability of the input BLC and pH data on the calculations of after maximal sprint and endurance exercise has not yet been investigated and was major aim of our study. Eleven subjects performed six maximal performance tests ranging from 20 s to 12 min duration over a 2-week period. All subjects were familiarized with the test conditions. For each performance test, pre and posttest BLC and pH was measured and used to calculate using the Henderson-Hasselbach equation. As BLC and pH values showed poor reliability, calculations were repeated using constants for BLC (1.23 mmol·L) and pH (7.426) chosen from the average values in the experimental data. Test-retest reliability for BLC (ICC: 0.12, 95% CI -0.49-0.65, n.s.) and pH (ICC: 0.40, 95% CI -0.22-0.79, n.s.) was poor, whereas BLC (ICC: 0.95, 95% CI 0.82-0.99, < 0.05) and pH (ICC: 0.89, 95% CI 0.65-0.97, < 0.05) displayed good to excellent reliability. Good reliability was observed for calculated from the Henderson-Hasselbalch equation utilizing BLC and pH only (ICC: 0.86, 95% CI 0.55-0.96, < 0.05). The validity of this method in comparison with gold-standard methods needs further scientific investigation.
传统上，缓冲能力（）是通过测量肌肉活检样本中肌肉pH值相对于标准化量盐酸暴露的变化来测定的。这是一种侵入性方法，需要特定设备和经过培训的人员，这限制了其在正常训练环境中的可用性。有人提出使用毛细血管血乳酸浓度（BLC）和pH值来评估，这是一种更实用且成本效益更高的方法。最大短跑和耐力运动后，输入的BLC和pH数据在计算中的可靠性尚未得到研究，这是我们研究的主要目的。11名受试者在2周内进行了6次持续时间从20秒到12分钟的最大性能测试。所有受试者都熟悉测试条件。对于每次性能测试，测量测试前后的BLC和pH值，并使用亨德森 - 哈塞尔巴赫方程计算。由于BLC和pH值显示出较差的可靠性，因此使用从实验数据平均值中选取的BLC常数（1.23 mmol·L）和pH常数（7.426）重复进行计算。BLC（组内相关系数：0.12，95%置信区间 -0.49 - 0.65，无显著性差异）和pH（组内相关系数：0.40，95%置信区间 -0.22 - 0.79，无显著性差异）的重测可靠性较差，而BLC（组内相关系数：0.95，95%置信区间 0.82 - 0.99，P < 0.05）和pH（组内相关系数：0.89，95%置信区间 0.65 - 0.97，P < 0.05）显示出良好到优秀的可靠性。仅使用BLC和pH通过亨德森 - 哈塞尔巴赫方程计算得到的显示出良好的可靠性（组内相关系数：0.86，95%置信区间 0.55 - 0.96，P < 0.05）。与金标准方法相比，该方法的有效性需要进一步的科学研究。
