This study focuses on implementing a composition of the natural flavonoid dihydroquercetin (DHQ) with L-lysine in the treatment of thermal burns. The wound-healing activity of DHQ is well-known. The addition of amino acid to the composition increases the water solubility of the flavonoids, providing an opportunity to develop a spray dosage form. The research involved 60 male Wistar rats divided into five treatment groups. Sea buck oil served as a positive control. On day 14, the composition treatment group showed significant progress in wound healing, being 9.6 ± 2.0% ahead of the other groups in absolute terms. On day 35, treatment with the composition resulted in a significant decrease in relative wound area to 1.9 ± 0.9%, while in the negative and positive control groups, it was 10.7 ± 7.8% and 8.4 ± 4.9%, respectively. At the same time, the epidermal and dermal layers were found to be clearly distinguished in the composition treatment according to histological analysis. Numerous collagen fibres were clearly visible, and the active process of keloid scar formation was observed. An additive effect of the combined use of DHQ and L-lysine was observed ( = 0.21, = 0.649). A natural next step is to develop the dosage form for the DHQ-L-lysine composition.