Miller T A, Li D, Kuo Y J, Schmidt K L, Shanbour L L
Am J Physiol. 1985 Jul;249(1 Pt 1):G137-44. doi: 10.1152/ajpgi.1985.249.1.G137.
By use of an in vivo canine chambered stomach preparation in which the gastric mucosa was partitioned into two equal halves, the effect of topical 16,16-dimethyl PGE2 (DMPGE2) (1 microgram/ml of perfusate) and 8% and 40% ethanol on tissue levels of nonprotein sulfhydryl compounds was assessed. Both DMPGE2 and 8% ethanol significantly increased (P less than 0.005) mucosal levels of nonprotein sulfhydryls when compared with corresponding mucosa bathed with saline alone. In contrast, mucosa bathed with 40% ethanol showed significantly decreased levels. If mucosa was bathed with DMPGE2 or 8% ethanol prior to exposing the stomach to 40% ethanol, this depletion in sulfhydryl compounds was not observed. Since other experimental observations have shown that exogenously administered prostaglandins and mild irritants (such as low-dose alcohol) can prevent gastric mucosal damage by necrotizing agents (such as high-dose alcohol), our findings are consistent with the hypothesis that nonprotein sulfhydryls may play a role in mediating gastric mucosal protection.
通过使用一种体内犬腔胃制备方法，即将胃黏膜分成两个相等的部分，评估了局部应用16,16 - 二甲基前列腺素E2（DMPGE2）（1微克/毫升灌注液）以及8%和40%乙醇对非蛋白巯基化合物组织水平的影响。与仅用盐水冲洗的相应黏膜相比，DMPGE2和8%乙醇均显著增加（P < 0.005）了黏膜中非蛋白巯基的水平。相反，用40%乙醇冲洗的黏膜显示水平显著降低。如果在将胃暴露于40%乙醇之前先用DMPGE2或8%乙醇冲洗黏膜，则未观察到巯基化合物的这种耗竭。由于其他实验观察表明，外源性给予的前列腺素和轻度刺激物（如低剂量酒精）可预防坏死剂（如高剂量酒精）引起的胃黏膜损伤，我们的发现与非蛋白巯基可能在介导胃黏膜保护中起作用的假设一致。
