Mizoue Tetsuya, Yamamoto Shohei, Tanaka Akihito, Oshiro Yusuke, Inamura Natsumi, Konishi Maki, Ozeki Mitsuru, Ohmagari Norio
Department of Epidemiology and Prevention, Center for Clinical Sciences, National Center for Global Health and Medicine, Toyko, Japan.
Department of Laboratory Testing, Center Hospital of the National Center for the Global Health and Medicine, Tokyo, Japan.
GHM Open. 2022 Aug 31;2(1):51-53. doi: 10.35772/ghmo.2021.01030.
The sensitivity of immunoassay for antibodies to SARS-CoV-2 may decrease over time. We compared three serology assays against SARS-CoV-2 nucleocapsid protein using serum samples of 20 health care workers with a history of mild PCR-confirmed coronavirus disease 2019 (COVID-19) at various timing since diagnosis. Overall, the sensitivity (95% confidence interval) was 95.0 (75.1-99.9), 60.0 (36.1-81.0), and 45.0 (23.1-68.5) for the Roche, Sysmex, and Abbott assays, respectively. The sensitivity of all these assays exceeded 80 for those diagnosed within 6 months, whereas it varied considerably for those diagnosed more than 6 months ago: 88.9 (Roche) versus 0 (Abbott). The present data provide a reference for researchers planning serological studies and interpreting these data. Such assay difference in terms of detection durability can be used, if used in combination, for the estimation of the timing of previously undetected infection.
针对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）抗体的免疫测定敏感性可能会随时间下降。我们使用20名医护人员的血清样本，针对SARS-CoV-2核衣壳蛋白比较了三种血清学检测方法，这些医护人员有轻度聚合酶链反应（PCR）确诊的2019冠状病毒病（COVID-19）病史，且处于确诊后的不同时间点。总体而言，罗氏、希森美康和雅培检测方法的敏感性（95%置信区间）分别为95.0（75.1-99.9）、60.0（36.1-81.0）和45.0（23.1-68.5）。对于在6个月内确诊的患者，所有这些检测方法的敏感性均超过80%，而对于6个月前确诊的患者，敏感性差异很大：罗氏检测为88.9%，雅培检测为0%。本数据为计划进行血清学研究和解释这些数据的研究人员提供了参考。如果联合使用，这种检测方法在检测持久性方面的差异可用于估计先前未检测到的感染时间。
