Wang Tianye, Wu Jiaqi, Nakagawa So, Yonezawa Takahiro, Liu Zhenqiu, Zhang Xin, Wang Haili, Li Yi, Zhang Tiejun
Department of Epidemiology, School of Public Health, Fudan University, Shanghai, 200032, China.
Department of Molecular Life Science, Tokai University School of Medicine, Isehara, Kanagawa, 259-1193, Japan.
Arch Virol. 2025 Mar 27;170(5):92. doi: 10.1007/s00705-025-06259-9.
With the widespread prevalence of Kaposi's sarcoma-associated herpesvirus (KSHV) globally, particularly in sub-Saharan Africa and the Mediterranean region, the open reading frame (ORF) K1 gene, a key gene for distinguishing different subtypes of KSHV, has been extensively studied for its diversity and sequence variations. In this study, we collected K1 gene sequences representing subtypes of KSHV worldwide in order to assess the global distribution of KSHV subtypes and to investigate the recombination and selection history of KSHV. Recombination and gene flow analysis indicated a minimum average recombination rate of 0.41 per site for the K1 gene. Recombination analysis indicated that 11 major recombination events had occurred, predominantly in subtypes A and C, while subtype B showed minimal involvement in recombination processes, consistent with the gene flow analysis. Using tip-dating methods, we estimated that the most recent common ancestor of KSHV emerged in the 12th century, while the currently globally prevalent subtypes appeared within the past three centuries. Its recent origin and rapid evolution indicate that KSHV is now undergoing strong selection and is in the process of adaptation.
随着卡波西肉瘤相关疱疹病毒（KSHV）在全球范围内的广泛流行，特别是在撒哈拉以南非洲和地中海地区，开放阅读框（ORF）K1基因作为区分KSHV不同亚型的关键基因，其多样性和序列变异已得到广泛研究。在本研究中，我们收集了代表全球KSHV亚型的K1基因序列，以评估KSHV亚型的全球分布，并研究KSHV的重组和选择历史。重组和基因流分析表明，K1基因每个位点的平均重组率最低为0.41。重组分析表明，已发生了11次主要重组事件，主要发生在A和C亚型中，而B亚型在重组过程中的参与最少，这与基因流分析结果一致。使用末端定年方法，我们估计KSHV的最近共同祖先出现在12世纪，而目前全球流行的亚型出现在过去三个世纪内。其近期起源和快速进化表明，KSHV目前正在经历强烈的选择，并且正处于适应过程中。
