Mehdipour Mahdieh, Mohammadi Hossein, Salih Sahar Abdalkarim, Rashidi Ali
Department of Agriculture, Iranian Research Organization for Science and Technology (IROST), Tehran, Iran.
Department of Animal Science, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran.
Sci Rep. 2025 Mar 27;15(1):10526. doi: 10.1038/s41598-025-95820-2.
Oxidative stress impairs sperm function and fertility in rams, most notably during cryopreservation, and is characterized by compromised sperm function and motility. Mitochondria contribute immensely towards sperm function, such as mitochondrial membrane potential (MMP) which is crucial for ATP production. The purpose of this study was to assess the role of MitoPBN supplementation on cryopreserved sperm quality in mature Ghezel ram sperm. Semen samples were treated with 0 (control), 100, 150, 200, and 250 µmol/L MitoPBN and then analyzed for key sperm factors. Results indicated that supplementation with 100 and 150 µmol/L MitoPBN significantly enhanced total motility, increasing to 52.36 ± 4.26% and 54.16 ± 3.19%, respectively. Progressive motility similarly improved to 24.82 ± 3.27% and 26.77 ± 3.46%, respectively. Additionally, sperm membrane integrity was markedly increased to 50.01 ± 4.22% and 52.54 ± 2.24%, while mitochondrial activity was significantly augmented from 35.14 ± 4.09% to 46.16 ± 4.02% and 50.26 ± 6.69%. Sperm viability also improved, rising to 48.99 ± 3.98% and 52.20 ± 3.17%, respectively. Notably, reactive oxygen species (ROS) levels were reduced to 2.95 ± 0.16% and 2.80 ± 0.11%, respectively, paralleled by enhancements in total antioxidant capacity (TAC; 1.85 ± 0.21% and 1.93 ± 0.16%, respectively) and glutathione peroxidase (GPx) activity (61.16 ± 4.77% and 63.36 ± 4.95%, respectively). Moreover, ATP content reached a peak of 116.29 ± 5.83 in the 150 µmol/L group. In conclusion, our results evidence that oxidative stress during ram sperm cryopreservation is effectively countered by MitoPBN, improving sperm quality.
氧化应激会损害公羊的精子功能和生育能力，在冷冻保存过程中尤为明显，其特征是精子功能和活力受损。线粒体对精子功能有巨大贡献，例如线粒体膜电位（MMP）对ATP生成至关重要。本研究的目的是评估补充线粒体靶向抗氧化剂MitoPBN对成熟盖泽尔公羊冷冻精子质量的作用。精液样本分别用0（对照）、100、150、200和250 µmol/L的MitoPBN处理，然后分析关键的精子因素。结果表明，补充100和150 µmol/L的MitoPBN可显著提高总活力，分别增至52.36±4.26%和54.16±3.19%。渐进性活力同样分别提高到24.82±3.27%和26.77±3.46%。此外，精子膜完整性显著提高到50.01±4.22%和52.54±2.24%，而线粒体活性从35.14±4.09%显著提高到46.16±4.02%和50.26±6.69%。精子活力也有所提高，分别升至48.99±3.98%和52.20±3.17%。值得注意的是，活性氧（ROS）水平分别降至2.95±0.16%和2.80±0.11%，同时总抗氧化能力（TAC；分别为1.85±0.21%和1.93±0.16%）和谷胱甘肽过氧化物酶（GPx）活性（分别为61.16±4.77%和63.36±4.95%）增强。此外，150 µmol/L组的ATP含量达到峰值116.29±5.83。总之，我们的结果证明，MitoPBN可有效对抗公羊精子冷冻保存过程中的氧化应激，提高精子质量。
