Belkova Natalia, Nemchenko Uliana, Klimenko Elizaveta, Smurova Nadezhda, Zugeeva Raisa, Sukhoreva Marina, Sinkov Viacheslav, Savilov Evgenij
Federal State Budgetary Scientific Institution 'Scientific Centre for Family Health and Human Reproduction Problems', Epidemiology and Microbiology Institute, 3, K. Marks Str., 664003 Irkutsk, Russia.
Regional State Autonomous Healthcare Institution 'Ivano-Matreninskaya City Children's Clinical Hospital', 57, Sovetskaya Str., 664009 Irkutsk, Russia.
Antibiotics (Basel). 2025 Mar 14;14(3):302. doi: 10.3390/antibiotics14030302.
is one of the leading causes of nosocomial respiratory tract infections, significantly affecting morbidity and mortality. It can persist in the lungs of patients with cystic fibrosis (CF) for extended periods because of its adaptive capacity. The main aim of this study was to determine the phenotypic and genotypic resistance to antibiotics of clinical isolates of that persist in patients with CF receiving long-term antimicrobial therapy. The study included nine strains of isolated from the sputum of patients with CF admitted to the hospital. Susceptibility to antibiotics was determined using the European Committee on Antimicrobial Susceptibility Testing (EUCAST) criteria. Whole-genome sequencing was performed for phylogeny, sequence typing, and to identify antibiotic-resistant genes. The study showed that during long-term persistence in the lungs of patients receiving antibacterial therapy, the restoration of susceptibility to antibiotics occurred in some cases. Multilocus sequence typing and phylogeny revealed six sequence types. Functional annotation identified 72 genes responsible for resistance to antibacterial and chemical substances, with either chromosomal or plasmid localisation.
是医院获得性呼吸道感染的主要原因之一，对发病率和死亡率有显著影响。由于其适应能力，它可在囊性纤维化（CF）患者的肺部长期存在。本研究的主要目的是确定在接受长期抗菌治疗的CF患者中持续存在的临床分离株对抗生素的表型和基因型耐药性。该研究纳入了从住院CF患者痰液中分离出的9株该菌。使用欧洲抗菌药物敏感性试验委员会（EUCAST）标准测定对抗生素的敏感性。进行全基因组测序以分析系统发育、序列分型并鉴定抗生素耐药基因。研究表明，在接受抗菌治疗的患者肺部长期存在期间，某些情况下会恢复对抗生素的敏感性。多位点序列分型和系统发育分析揭示了六种序列类型。功能注释确定了72个负责对抗菌和化学物质耐药的基因，这些基因位于染色体或质粒上。
