梅氏珊瑚线粒体全基因组及其线粒体基因组特征分析

Complete Mitochondrial Genome of and Characteristics Analysis of the Mitochondrial Genome in Merulinidae.

作者信息

Jia Shuwen, Shen Tongtong, Cai Wenqi, Zhang Jian, Chen Shiquan

机构信息

Qukou Scientific Research Base, Institute of Marine Ecology, Hainan Academy of Ocean and Fisheries Sciences, Haikou 571136, China.

Key Laboratory for Coastal Marine Eco-Environment Process and Carbon Sink of Hainan Province, Yazhou Bay Innovation Institute, College of Ecology and Environment, Hainan Tropical Ocean University, Sanya 572000, China.

出版信息

Genes (Basel). 2025 Mar 2;16(3):304. doi: 10.3390/genes16030304.

DOI:10.3390/genes16030304

PMID:40149455

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11942114/

Abstract

BACKGROUND

The Merulinidae family belonging to the order Scleractinia is mainly distributed in the Indo-Pacific and Caribbean regions and often constitute the most dominant species of coral reefs. Mitochondrial genome is a key tool for studying the phylogeny and adaptation. Only a few studies have conducted the characteristics analyses of mitochondrial genome in the Merulinidae family.

METHODS

Therefore, we used high-throughput sequencing technology to describe the mitochondrial genome of , a member of this family. Bioinformatics was used to analyze the composition characteristics of the mitochondrial genome of 10 Merulinidae species.

RESULTS

The mitochondrial genome of had a total length of 16,462 bp and a GC content of 33.0%. Thirteen unique protein-coding genes (PCGs), two transfer RNA (tRNA) genes, and two ribosomal RNA (rRNA) genes were annotated. Each species of Merulinidae had 13 unique PCGs in the mitochondrial genome. In contrast, the number of tRNAs and rRNAs significantly varied in Merulinidae species. Collinearity and gene rearrangement analyses indicated that the mitochondrial evolution of species in the Merulinidae family was relatively conserved. Divergence time analysis indicated that Merulinidae originated in the Oligocene, whereas the genus originated in the Miocene. The formation and intraspecific divergence of coral species were consistent with geological changes in the ocean.

CONCLUSIONS

The results of this study help better understand the characteristics of the mitochondrial genome in the Merulinidae family and provide insights into the utility of mitochondrial genes as molecular markers of phylogeny.

摘要

背景

属于石珊瑚目（Scleractinia）的裸肋珊瑚科（Merulinidae）主要分布在印度-太平洋和加勒比地区，并且常常构成珊瑚礁中最具优势的物种。线粒体基因组是研究系统发育和适应性的关键工具。仅有少数研究对裸肋珊瑚科的线粒体基因组进行了特征分析。

方法

因此，我们使用高通量测序技术来描述该科成员的线粒体基因组。利用生物信息学分析了10种裸肋珊瑚科物种线粒体基因组的组成特征。

结果

的线粒体基因组全长16,462 bp，GC含量为33.0%。注释出了13个独特的蛋白质编码基因（PCGs）、2个转运RNA（tRNA）基因和2个核糖体RNA（rRNA）基因。裸肋珊瑚科的每个物种在线粒体基因组中都有13个独特的PCGs。相比之下，tRNAs和rRNAs的数量在裸肋珊瑚科物种中显著不同。共线性和基因重排分析表明，裸肋珊瑚科物种的线粒体进化相对保守。分歧时间分析表明，裸肋珊瑚科起源于渐新世，而属起源于中新世。珊瑚物种的形成和种内分歧与海洋地质变化一致。