Hagita Yujiro, Miura Ryotaro, Shirasuna Koumei, Ajito Tadaharu, Matsumoto Hirotaka
Fuji Animal Farm, Nippon Veterinary and Life Science University, 799 Fujikane, Kawaguchiko-cho, Minamitsuru-gun 401-0338, Yamanashi, Japan.
School of Veterinary, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, Kyonan-cho, Musashino-shi 180-8602, Tokyo, Japan.
Animals (Basel). 2025 Mar 7;15(6):767. doi: 10.3390/ani15060767.
This study aimed to investigate the effects of oxidative stress and antioxidant activity in plasma and uterine fluid during early postpartum on the reproductive performance of Japanese Black cows. A total of 17 Japanese Black cows were included in this study. Plasma and uterine fluid samples were collected at days 7 and 14 of the estrus cycle (n = 17), between 47-67 days postpartum, and diacron-reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) were measured. d-ROMs, BAP, and oxidation stress index (OSI: d-ROMs/BAP × 100) were compared between pregnant and nonpregnant cows at first postpartum artificial insemination (AI), cows pregnant less than 120 days postpartum (<120 days postpartum), and cows pregnant more than 120 days postpartum (≥120 days postpartum) regardless of AI number. The uterine fluid OSI on day 7 of the estrus cycle was significantly higher in cows pregnant ≥ 120 days postpartum ( < 0.05). d-ROMs, BAP, and OSI of uterine fluid during early postpartum were useful indicators for determining the subsequent reproductive performance of Japanese Black cows.
本研究旨在探讨产后早期血浆和子宫液中的氧化应激及抗氧化活性对日本黑牛繁殖性能的影响。本研究共纳入17头日本黑牛。在发情周期的第7天和第14天（n = 17）、产后47 - 67天采集血浆和子宫液样本，并测定二烯共轭反应性氧代谢产物（d-ROMs）和生物抗氧化能力（BAP）。比较首次产后人工授精（AI）时怀孕和未怀孕的母牛、产后怀孕少于120天（产后<120天）的母牛以及产后怀孕超过120天（产后≥120天）的母牛（不考虑AI次数）之间的d-ROMs、BAP和氧化应激指数（OSI：d-ROMs/BAP×100）。产后≥120天怀孕的母牛发情周期第7天的子宫液OSI显著更高（<0.05）。产后早期子宫液的d-ROMs、BAP和OSI是确定日本黑牛后续繁殖性能的有用指标。
