Hou Yanling, Ye Huan, Yue Huamei, Li Junyi, Huang Ling, Qu Ziling, Ruan Rui, Lin Danqing, Liang Zhiqiang, Xie Yong, Li Chuangju
College of Fisheries and Life Sciences, Shanghai Ocean University, Shanghai 201306, China.
Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan 430223, China.
Animals (Basel). 2025 Mar 8;15(6):770. doi: 10.3390/ani15060770.
The largefin longbarbel catfish (), a freshwater species endemic to China with fundamental economic importance, requires investigation into its genetic structure for effective management. In this study, we employed mitochondrial cytochrome () gene sequences and 14 microsatellite loci to elucidate the genetic structure of 195 individuals across eight distinct populations. The analysis revealed a haplotype number (H) of 31, haplotype diversity (Hd) of 0.853, and nucleotide diversity (π) of 0.0127. Population neutrality tests indicated that Tajima's D (-0.59467) and Fu and Li's D* (0.56621) were not statistically significant, and the mismatch distribution exhibited a multimodal pattern. Microsatellite analysis revealed that the mean number of alleles (Na), observed heterozygosity (Ho), and polymorphic information content (PIC) across all loci were 18.500, 0.761, and 0.808, respectively. The UPGMA phylogram constructed based on genetic distance identified two distinct clusters, with paired Fst values ranging from 0.108 to 0.138. These results suggest that the largefin longbarbel catfish is in a state of dynamic equilibrium with high genetic diversity. Furthermore, there was significant genetic differentiation between the YB population and the other seven populations, indicating that the population in the upper reaches of the Yangtze River should be managed as a distinct unit.
大鳍鳠是中国特有的具有重要经济价值的淡水物种，为实现有效管理，需要对其遗传结构进行研究。在本研究中，我们利用线粒体细胞色素 基因序列和14个微卫星位点，阐明了8个不同种群中195个个体的遗传结构。分析结果显示，单倍型数量（H）为31，单倍型多样性（Hd）为0.853，核苷酸多样性（π）为0.0127。群体中性检验表明， Tajima's D（-0.59467）和Fu and Li's D*（0.56621）无统计学意义，错配分布呈现多峰模式。微卫星分析显示，所有位点的平均等位基因数（Na）、观察杂合度（Ho）和多态信息含量（PIC）分别为18.500、0.761和0.808。基于遗传距离构建的UPGMA系统发育树识别出两个不同的聚类，成对Fst值范围为0.108至0.138。这些结果表明，大鳍鳠处于动态平衡状态，具有较高的遗传多样性。此外，YB种群与其他七个种群之间存在显著的遗传分化，这表明长江上游的种群应作为一个独立单元进行管理。
