肽抗生素乳链菌肽的作用模式及其对全细胞膜电位以及细胞质膜和人工膜囊泡的影响。
Mode of action of the peptide antibiotic nisin and influence on the membrane potential of whole cells and on cytoplasmic and artificial membrane vesicles.
作者信息
Ruhr E, Sahl H G
出版信息
Antimicrob Agents Chemother. 1985 May;27(5):841-5. doi: 10.1128/AAC.27.5.841.
Abstract
The peptide antibiotic nisin was shown to cause a rapid efflux of amino acids and Rb+ from the cytoplasm of gram-positive bacteria (Staphylococcus cohnii 22, Bacillus subtilis W 23, Micrococcus luteus ATCC 4698, and Streptococcus zymogenes 24). It strongly decreased the membrane potential of cells as judged by the distribution of the lipophilic tetraphenylphosphonium cation. Ascorbate-phenazine methosulfate-driven transport of L-proline by cytoplasmic membrane vesicles was blocked after addition of nisin, and accumulated amino acids were released from the vesicles. Soybean phospholipid (asolectin) vesicles were not affected by nisin. The data suggest that the cytoplasmic membrane is the primary target and that membrane disruption accounts for the bactericidal action of nisin.
摘要
肽抗生素乳链菌肽可使革兰氏阳性菌（科氏葡萄球菌22、枯草芽孢杆菌W23、藤黄微球菌ATCC 4698和发酵链球菌24）细胞质中的氨基酸和Rb+快速外流。通过亲脂性四苯基鏻阳离子的分布判断，它能显著降低细胞的膜电位。添加乳链菌肽后，抗坏血酸 - 吩嗪硫酸甲酯驱动的细胞质膜囊泡对L - 脯氨酸的转运被阻断，囊泡中积累的氨基酸被释放出来。大豆磷脂（大豆卵磷脂）囊泡不受乳链菌肽影响。这些数据表明，细胞质膜是主要靶点，膜破坏是乳链菌肽杀菌作用的原因。
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