Collawn J F, Fish W W
Arch Biochem Biophys. 1985 Jul;240(1):242-5. doi: 10.1016/0003-9861(85)90029-3.
The out-exchange kinetics of tritium from apoferritin, ferritin of various iron contents, and apoferritin subunits were examined. The exchange kinetics indicated no detectable conformational differences in the tetracosamer with and without hydrous ferric oxide in the internal cavity of the molecule. The data for apoferritin subunits were markedly different from those for the tetracosameric state. The exchange kinetics for apoferritin were consistent with a rapid exchange of water between the internal cavity of the protein and the bulk solvent outside the protein shell.
研究了脱铁铁蛋白、不同铁含量的铁蛋白以及脱铁铁蛋白亚基中氚的外交换动力学。交换动力学表明，分子内腔中有无水合氧化铁的二十四聚体在构象上没有可检测到的差异。脱铁铁蛋白亚基的数据与二十四聚体状态的数据明显不同。脱铁铁蛋白的交换动力学与蛋白质内腔与蛋白质外壳外的大量溶剂之间水的快速交换一致。
