胡芦菔汤对环磷酰胺诱导的小鼠自身免疫性早发性卵巢功能不全模型的影响。
Effects of Huluan decotion on cyclophosphamide-induced autoimmune premature ovarian failure in murine models.
作者信息
Guiling Feng, Xiaolin Zhou, Chengwan Shen, Panxiao L I, Abudula Abulizi
机构信息
College of Medicial science, Ningde Normal university, Ningde 352000, China.
2 Fujian Key Laboratory of Toxicology and Pharmacotoxicology, Ningde 352000, China.
出版信息
J Tradit Chin Med. 2025 Apr;45(2):266-271. doi: 10.19852/j.cnki.jtcm.20240927.001.
OBJECTIVES
To investigate the therapeutic effect of Huluan decotion (, HLD) on cyclophosphamide-induced premature ovarian failure (POF) in mice and its regulatory mechanisms.
METHODS
Female BALB/c mice were administered cyclophosphamide and administered received different doses of HLD for 28 d. Levels of sex hormone, such as estradiol (E2), follicle stimulating hormone (FSH) and luteinizing hormone (LH) in the sera, were assessed using enzyme-linked immunosorbent assay (ELISA). Follicular structure variances were observed through hematoxylin and eosin (HE) staining, while Forkhead box L2 (FOXL2) expression were analyzed immune-ohistochemical staining. The primary mechanism of POF were investigated through Western blot analysis.
RESULTS
E2 levels decreased, and FSH and LH levels increased in POF model mice, but these trends were reversed with HLD or premarin administration, the expressions of WNT family member 4 (Wnt4), β-Catenin and FOXL2 were downregulated in POF model mice, whereas high expression levels were observed in control mice and other groups.
CONCLUSION
HLD effectively treats POF induced with cyclophosphamide in mice by enhancing expressions of Wnt4, β-Catenin and FOXL2.
目的
探讨护卵汤（HLD）对环磷酰胺诱导的小鼠卵巢早衰（POF）的治疗作用及其调控机制。
方法
对雌性BALB/c小鼠给予环磷酰胺，并给予不同剂量的HLD，持续28天。采用酶联免疫吸附测定（ELISA）法评估血清中雌二醇（E2）、卵泡刺激素（FSH）和黄体生成素（LH）等性激素水平。通过苏木精-伊红（HE）染色观察卵泡结构变化，同时采用免疫组织化学染色分析叉头框L2（FOXL2）表达。通过蛋白质印迹分析研究POF的主要机制。
结果
POF模型小鼠中E2水平降低，FSH和LH水平升高，但给予HLD或结合雌激素后这些趋势得到逆转，POF模型小鼠中WNT家族成员4（Wnt4）、β-连环蛋白和FOXL2的表达下调，而在对照小鼠和其他组中观察到高表达水平。
结论
HLD通过增强Wnt4、β-连环蛋白和FOXL2的表达有效治疗环磷酰胺诱导的小鼠POF。
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