Sheng Wentao
Department of Biological Technology, Nanchang Normal University, Nanchang, 330032, Jiangxi, China.
Open Life Sci. 2025 Mar 25;20(1):20251070. doi: 10.1515/biol-2025-1070. eCollection 2025.
(L.) Cass., 1820 of the genus is of importance in Chinese ethnic medicine. In this research, the whole genome DNA of was extracted and sequenced using the Illumina NovaSeq platform, its chloroplast genome was assembled and annotated, and its sequence characteristics were analyzed using bioinformatic methods. The results showed that its chloroplast genome has a length of 151,871 bp and contains 133 annotated genes, consisting of 88 protein-coding genes, 8 rRNA genes, and 37 tRNA genes. In total, 202 simple sequence repeat sites and 43 long repeats were detected in , mainly consisting of mono-nucleotide and tri-nucleotide repeats, with A/T as the major base composition. The chloroplast genome of contains 22,772 codons, with leucine-coding codons being the most abundant. Comparative genomics showed that the genome structure, composition and variation were basically the same in the Asteraceae family. The phylogenetic tree analysis indicated a close relationship between the genus and consistent with the morphological classification. The research of the chloroplast genome will lay a foundation for species discrimination, genetic evolution analysis, and DNA barcode construction in plants.
（L.）卡斯., 1820年的该属植物在中国民族医学中具有重要意义。在本研究中，使用Illumina NovaSeq平台提取并测序了该植物的全基因组DNA，组装并注释了其叶绿体基因组，并使用生物信息学方法分析了其序列特征。结果表明，其叶绿体基因组长度为151,871 bp，包含133个注释基因，由88个蛋白质编码基因、8个rRNA基因和37个tRNA基因组成。总共在该植物中检测到202个简单序列重复位点和43个长重复序列，主要由单核苷酸和三核苷酸重复组成，以A/T作为主要碱基组成。该植物的叶绿体基因组包含22,772个密码子，其中亮氨酸编码密码子最为丰富。比较基因组学表明，菊科植物的基因组结构、组成和变异基本相同。系统发育树分析表明该属植物与[另一属]关系密切，与形态学分类一致。该植物叶绿体基因组的研究将为该植物的物种鉴别、遗传进化分析和DNA条形码构建奠定基础。
