Chau Katherine D, Crone Makaylee K, Nguyen Phuong N, Rehan Sandra M
Department of Biology, York University, Toronto, Canada.
Environ Microbiol. 2025 Apr;27(4):e70089. doi: 10.1111/1462-2920.70089.
We used flowers to explore how ephemeral anthosphere microbiomes differ among flowering plant species and along an urban gradient. Here, we sequenced 16S rRNA for bacteria, ITS1 for fungi and rbcL for plant DNA from 10 different plant species sampled to characterise anthosphere microbiomes along an urban gradient and identify important network interactions. Bacterial and fungal flower microbiomes significantly differed in diversity across plant species, especially among Asteraceae and Fabaceae. Across all analyses, four taxa, the bacteria Pantoea and Rosenbergiella and the fungi Alternaria and Cladosporium were highly prevalent and contributed to the majority of microbiome composition differences observed between plant species. These four taxa harbour strains or species that may be either pathogenic or beneficial to plants. Across a land use gradient, the plant community bacterial and fungal microbiome was stable and consistent. Flower-plant networks confirmed all focal flower families in abundance on each sampled flower, with the addition of Paulowniaceae, suggesting that pollinators visiting the focal flowers also visit this plant family. Our findings reveal that anthosphere microbiomes are diverse at the plant community level and encouragingly remain robust against urbanisation.
我们利用花朵来探究短暂存在的花osphere微生物群在开花植物物种之间以及沿城市梯度是如何不同的。在这里，我们对从10种不同植物物种中采集的样本进行了细菌16S rRNA测序、真菌ITS1测序以及植物DNA的rbcL测序，以描绘沿城市梯度的花osphere微生物群，并识别重要的网络相互作用。细菌和真菌的花朵微生物群在不同植物物种间的多样性存在显著差异，尤其是在菊科和豆科之间。在所有分析中，四个分类群，即细菌泛菌属和罗森伯格菌属以及真菌链格孢属和枝孢属高度普遍，并且导致了观察到的植物物种间微生物群组成差异的大部分。这四个分类群包含可能对植物致病或有益的菌株或物种。在土地利用梯度上，植物群落的细菌和真菌微生物群是稳定且一致的。花卉-植物网络证实了每个采样花朵上所有重点花卉科的丰富度，还新增了泡桐科，这表明访问重点花卉的传粉者也会访问这个植物科。我们的研究结果表明，花osphere微生物群在植物群落水平上是多样的，并且令人鼓舞的是，它们对城市化具有很强的抵抗力。
