Sariçam Esma, Altınışık Hanife, Bulak Neslihan, Güven Berna, Yurdagül Sabiha Zeynep, Amasya Gülin
Department of Endodontics, Faculty of Dentistry, Ankara Yıldırım Beyazıt University, Turkey.
Department of Restorative Dentistry, Faculty of Dentistry, Gazi University, Ankara, Turkey.
Dent Med Probl. 2025 Jul-Aug;62(4):731-737. doi: 10.17219/dmp/167467.
Chitosan prepared with acetic acid is commonly used as an endodontic irrigant. However, the chitosan solution prepared with distilled water has not been evaluated for endodontic usage.
The present study aimed to compare the effects of ethylenediaminetetraacetic acid (EDTA), fumaric acid, and the chitosan solutions prepared with distilled water (C-DW) and acetic acid (C-AA) on dentin microhardness and dentinal tubule penetration.
Eighty maxillary central incisors were endodontically instrumented and randomly divided into 2 main groups (n = 40) for the evaluation of dentin microhardness and tubular penetration, with 4 subgroups in each main group (n = 10) according to the final irrigation solutions used (EDTA, fumaric acid, C-DW, and C-AA). The C-AA solution was prepared by diluting medium-molecularweight chitosan in acetic acid. The C-DW solution was prepared with distilled water and chitosan ammonium salts, which were synthesized using trichloroacetic acid and low-molecular-weight chitosan. After irrigation, the roots were sectioned horizontally 2 mm (the apical third) and 5 mm (the middle third) from the apex. The microhardness measurements were taken at depths of 500 μm and 1,000 μm from the canal lumen. The sections were examined for tubular penetration using confocal laser scanning microscopy. The data was analyzed using the analysis of variance (ANOVA), with a significance level set at p < 0.05.
The microhardness values were statistically similar at either depth for each third (p > 0.05), except for the 1,000-μm depth in the apical third, where the use of the C-AA solution resulted in lower microhardness as compared to fumaric acid (p < 0.05). No significant differences were observed in tubular penetration with regard to each third (p > 0.05).
All solutions showed a similar penetration ability in each third. At the 1,000-μm depth in the apical third, the fumaric acid solution provided a higher microhardness value than the C-AA solution.
用乙酸制备的壳聚糖通常用作根管冲洗剂。然而，用蒸馏水制备的壳聚糖溶液尚未进行根管治疗用途的评估。
本研究旨在比较乙二胺四乙酸（EDTA）、富马酸以及用蒸馏水（C-DW）和乙酸（C-AA）制备的壳聚糖溶液对牙本质显微硬度和牙本质小管渗透的影响。
选取80颗上颌中切牙进行根管预备，随机分为2个主要组（每组n = 40），分别用于评估牙本质显微硬度和小管渗透，每个主要组根据最终使用的冲洗液（EDTA、富马酸、C-DW和C-AA）再分为4个亚组（每组n = 10）。C-AA溶液是通过将中分子量壳聚糖稀释于乙酸中制备而成。C-DW溶液是用蒸馏水和壳聚糖铵盐制备的，壳聚糖铵盐是用三氯乙酸和低分子量壳聚糖合成的。冲洗后，将牙根从根尖水平切成2 mm（根尖三分之一处）和5 mm（根中三分之一处）的切片。在距根管腔500μm和1000μm深度处进行显微硬度测量。使用共聚焦激光扫描显微镜检查切片的小管渗透情况。数据采用方差分析（ANOVA）进行分析，显著性水平设定为p < 0.05。
除根尖三分之一处1000μm深度外，各三分之一处任一深度的显微硬度值在统计学上均相似（p > 0.05），在该深度，与富马酸相比，使用C-AA溶液导致显微硬度较低（p < 0.05）。各三分之一处的小管渗透情况未观察到显著差异（p > 0.05）。
所有溶液在各三分之一处均表现出相似的渗透能力。在根尖三分之一处1000μm深度，富马酸溶液提供的显微硬度值高于C-AA溶液。
