Liu Xin, Niu Shiyue, Yang Bin, Liu Jia, Niu Liqian, Wang Xian, Song Daqian, Bi Shuyun
College of Chemistry, Changchun Normal University, Changchun, 130032, China.
College of Chemistry, Jilin Province Research Center for Engineering and Technology of Spectral Analytical Instruments, Jilin University, Qianjin Street 2699, Changchun, 130012, China.
Talanta. 2025 Sep 1;292:128002. doi: 10.1016/j.talanta.2025.128002. Epub 2025 Mar 22.
Ultrasensitive Surface-enhanced Raman spectroscopy (SERS) method for the detection of diquat/dipterex was established using bovine serum albumin (BSA)-protected silver nanoparticles (AgNPs) modified MIL-53(Al) (named as BSA/MIL-53(Al)/AgNPs). Compared with unmodified AgNPs, BSA/MIL-53(Al)/AgNPs significantly enhanced the Raman signals of diquat and dipterex and the enhancement factors (EFs) were 1.58 × 10 and 2.34 × 10, respectively. The TEM, XRD, TGA, XPS, UV-vis and FT-IR were utilized to characterize BSA/MIL-53(Al)/AgNPs and the binding of the substrate with diquat/dipterex. The optimal measurement conditions were investigated in detail by single factor experiment and response surface model. The impacts of common pesticides and coexisting substances on the determination of diquat/dipterex were studied. Under optimum conditions, linear calibration curves for detecting diquat/dipterex were established with a limit of detection (LOD) of 0.17/0.89 pmol L (3S/S). The SERS approaches were used to detect diquat and dipterex in several fruits and vegetables. The recovery was 97.10 %-104.82 % with the relative standard deviation (RSD) of 1.04 %-4.15 % (n = 5).
利用牛血清白蛋白（BSA）保护的银纳米颗粒（AgNPs）修饰的MIL-53（Al）（命名为BSA/MIL-53（Al）/AgNPs）建立了用于检测敌草快/敌百虫的超灵敏表面增强拉曼光谱（SERS）方法。与未修饰的AgNPs相比，BSA/MIL-53（Al）/AgNPs显著增强了敌草快和敌百虫的拉曼信号，增强因子（EFs）分别为1.58×10和2.34×10。利用透射电子显微镜（TEM）、X射线衍射（XRD）、热重分析（TGA）、X射线光电子能谱（XPS）、紫外可见光谱（UV-vis）和傅里叶变换红外光谱（FT-IR）对BSA/MIL-53（Al）/AgNPs以及底物与敌草快/敌百虫的结合进行了表征。通过单因素实验和响应面模型详细研究了最佳测量条件。研究了常见农药和共存物质对敌草快/敌百虫测定的影响。在最佳条件下，建立了检测敌草快/敌百虫的线性校准曲线，检测限（LOD）为0.17/0.89 pmol L（3S/S）。采用SERS方法检测了几种水果和蔬菜中的敌草快和敌百虫。回收率为97.10%-104.82%，相对标准偏差（RSD）为1.04%-4.15%（n=5）。
