Zhao Hongcui, Wu Juan, Xu Yang, Shen Xiaofang, Wang Huanhuan, Zhao Aihua, Cao Fumin, Chen Xinna
Center of Reproductive Medicine, Beijing Amcare Beisanhuan Women's & Children's Hospital, Beijing, People's Republic of China.
J Ovarian Res. 2025 Mar 28;18(1):64. doi: 10.1186/s13048-025-01646-x.
The management of poor responders is a significant challenge for both patients and clinicians. The aim of this study was to evaluate the effectiveness of intra-ovarian injection of Platelet-Rich Plasma (PRP) combined with successive accumulated embryo transfer in improving the outcomes of patients with Poor Ovarian Response(POR) based on POSEIDON criteria.
This single-center, retrospective before-after study was conducted at a private reproductive center, involving 49 women diagnosed with POR, indicated by an AMH level of less than 1.2 ng/ml. The participants, comprising 13 group 3 and 36 group 4 POR patients, underwent intra-ovarian injections of PRP followed by the accumulation of embryos over three successive cycles of mild stimulation IVF/ICSI from May 2021 to May 2022, before proceeding to the embryo transfer phase. The ovarian reserve markers, oocyte and embryologic outcomes were compared in all patients before and after intra-ovarian injection of PRP. The cumulative clinical pregnancy and cumulative live birth outcomes were presented. Statistical analyses were performed using SPSS version 25. A p-value < 0.05 denoted statistical significance.
RESULT(S): The mean age of all participants was 37.67 ± 4.15 years and their mean body mass index was 21.52 ± 2.80 kg/m. Autologous intraovarian PRP therapy significantly increased AMH levels, AFC and decreased FSH levels. Autologous intraovarian PRP therapy accompanied with 3 successive cumulated cycles, significantly increased No. of accumulated embryos and blastocysts. This strategy also significantly reduced the rate of cancelled cycle. Following this strategy, of 44 cases with accumulated embryos/blastocysts transfer, 20 (45.45%) achieved clinical pregnancy, of which 15 (34.09%) resulted in live births and 5 (11.36%) ended in miscarriage.
CONCLUSION(S): Intra-ovarian injection of PRP plus successive embryo accumulation following mild stimulation and accumulated embryo transfer appears to be an optimal strategy for POR management.
Not applicable.
对于患者和临床医生而言，对卵巢反应不良者的管理都是一项重大挑战。本研究的目的是基于波塞冬标准，评估卵巢内注射富血小板血浆（PRP）联合连续累积胚胎移植在改善卵巢反应不良（POR）患者结局方面的有效性。
本单中心回顾性前后对照研究在一家私立生殖中心开展，纳入49名诊断为POR的女性，其抗缪勒管激素（AMH）水平低于1.2 ng/ml。参与者包括13名3组和36名4组POR患者，在2021年5月至2022年5月期间，先接受卵巢内PRP注射，然后在三个连续的轻度刺激体外受精/卵胞浆内单精子注射（IVF/ICSI）周期中累积胚胎，之后进入胚胎移植阶段。比较所有患者在卵巢内注射PRP前后的卵巢储备指标、卵母细胞和胚胎学结局。呈现累积临床妊娠和累积活产结局。使用SPSS 25版进行统计分析。p值<0.05表示具有统计学意义。
所有参与者的平均年龄为37.67±4.15岁，平均体重指数为21.
