评估卵巢内富血小板血浆（PRP）给药对卵巢反应不良患者卵母细胞相关变量的影响：根据 POSEIDON 标准的回顾性研究。
Evaluation of intra-ovarian platelet-rich plasma administration on oocytes-dependent variables in patients with poor ovarian response: A retrospective study according to the POSEIDON criteria.
机构信息
Endometrium and Endometriosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
出版信息
Reprod Biol Endocrinol. 2021 Sep 8;19(1):137. doi: 10.1186/s12958-021-00826-w.
BACKGROUND
Poor ovarian response (POR) is among the common findings in infertile women with no significant underlying condition. The aim of this study was to investigate the intra-ovarian potential of platelet-rich plasma (PRP) administration on oocytes-dependent variables in the POR women grouped according to the POSEIDON criteria.
METHODS
This retrospective study was performed on POR women with no underlying condition who have undergone intra-ovarian PRP injection. As well as patients' age, the number of total and MI, MII, and GV oocytes were extracted from the files. The laboratory variables including anti-mullerian hormone (AMH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol were also gathered. In order to reduce any bias due to the possible differences in kits or devices, a single laboratory with the highest number of cases was selected and others were excluded from the study. Then, the included cases were grouped into four according to the POSEIDON criteria and analyzed for the mentioned variables by SPSS, version 25. The statistical significance level was set as P-value < 0.05.
RESULTS
From 383 cases, a total number of 96 women were enrolled in this study. According to the POSEIDON criteria, group 4 (Age ≥ 35 years, AMH < 1.2 ng/mL) with the ratio of 56/96 (58.3%) had the highest prevalence among others. As the analyses showed, changes in the laboratory variables (LH, FSH, AMH, and estradiol) were not significant in almost all the groups following the intervention. Regarding the total oocytes number, PRP administration caused a significant increase in the total number in all the groups (all P < 0.05). Also, the number of MII oocytes was significantly increased following the treatment in all groups except for group 2 (Age ≥ 35 years, AMH ≥ 1.2 ng/mL; all P < 0.05). Of 96 cases, 14 (14.6%) got clinically pregnant following assisted reproductive techniques which this number were significantly differed among the groups (P = 0.002).
CONCLUSION
This study showed that PRP treatment was effective on total and MII oocyte numbers in the patients with POR, however, further studies are required.
背景
在没有明显潜在疾病的不孕女性中，卵巢反应不良（POR）是常见的发现。本研究的目的是根据 POSEIDON 标准，调查富含血小板的血浆（PRP）给药对 POR 女性卵母细胞相关变量的卵巢内潜力。
方法
这项回顾性研究针对没有潜在疾病且接受过卵巢内 PRP 注射的 POR 女性进行。从档案中提取患者年龄、总和 MI、MII 和 GV 卵母细胞数量。还收集了实验室变量，包括抗苗勒管激素（AMH）、卵泡刺激素（FSH）、黄体生成素（LH）和雌二醇。为了减少由于试剂盒或设备可能存在的差异而产生的任何偏差，选择了一个拥有最多病例的单一实验室，并将其他实验室排除在研究之外。然后，根据 POSEIDON 标准将纳入的病例分为四组，并使用 SPSS 版本 25 对所述变量进行分析。统计显著性水平设为 P 值<0.05。
结果
在 383 例中，共有 96 例女性纳入本研究。根据 POSEIDON 标准，比率为 56/96（58.3%）的第 4 组（年龄≥35 岁，AMH<1.2ng/mL）患病率最高。分析表明，干预后几乎所有组的实验室变量（LH、FSH、AMH 和雌二醇）变化均不显著。关于总卵母细胞数量，PRP 给药在所有组中均导致总数量显著增加（均 P<0.05）。此外，除第 2 组（年龄≥35 岁，AMH≥1.2ng/mL；均 P<0.05）外，所有组的 MII 卵母细胞数量在治疗后均显著增加。在 96 例患者中，有 14 例（14.6%）通过辅助生殖技术获得临床妊娠，而这一数字在各组之间存在显著差异（P=0.002）。
结论
本研究表明，PRP 治疗对 POR 患者的总卵母细胞和 MII 卵母细胞数量有效，但需要进一步研究。
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