Fresh non-fruit latex of Carica papaya contains papain, multiple forms of chymopapain A and papaya proteinase omega.
作者信息
Brocklehurst K, Salih E, McKee R, Smith H
出版信息
Biochem J. 1985 Jun 1;228(2):525-7. doi: 10.1042/bj2280525.
Abstract
摘要
相似文献
1
Fresh non-fruit latex of Carica papaya contains papain, multiple forms of chymopapain A and papaya proteinase omega.新鲜的番木瓜非果实乳胶含有木瓜蛋白酶、多种形式的木瓜凝乳蛋白酶A和木瓜蛋白酶ω。
Biochem J. 1985 Jun 1;228(2):525-7. doi: 10.1042/bj2280525.
2
The thiol proteinases from the latex of Carica papaya L. I. Fractionation, purification and preliminary characterization.番木瓜乳汁中的硫醇蛋白酶。I. 分级分离、纯化及初步特性分析。
Biol Chem Hoppe Seyler. 1988 Aug;369(8):733-40. doi: 10.1515/bchm3.1988.369.2.733.
3
Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex.
Biochem J. 1989 Jul 15;261(2):469-76. doi: 10.1042/bj2610469.
4
The thiol proteinases from the latex of Carica papaya L. IV. Proteolytic specificities of chymopapain and papaya proteinase omega determined by digestion of alpha-globin chains.
Biol Chem Hoppe Seyler. 1989 Aug;370(8):819-29. doi: 10.1515/bchm3.1989.370.2.819.
5
The thiol proteinases from the latex of Carica papaya L. III. The primary structure of chymopapain.番木瓜乳胶中的硫醇蛋白酶III. 木瓜凝乳蛋白酶的一级结构
Biol Chem Hoppe Seyler. 1989 May;370(5):425-34. doi: 10.1515/bchm3.1989.370.1.425.
6
A necessary modification to the preparation of papain from any high-quality latex of Carica papaya and evidence for the structural integrity of the enzyme produced by traditional methods.对从任何优质番木瓜乳胶制备木瓜蛋白酶的必要改进以及传统方法生产的酶结构完整性的证据。
Biochem J. 1979 Feb 1;177(2):541-8. doi: 10.1042/bj1770541.
7
The thiol proteinases from the latex of Carica papaya L. II. The primary structure of proteinase omega.番木瓜乳汁中的硫醇蛋白酶II. 蛋白酶ω的一级结构
Biol Chem Hoppe Seyler. 1988 Aug;369(8):741-54. doi: 10.1515/bchm3.1988.369.2.741.
8
Isolation and preliminary characterization of the cysteine-proteinases from the latex of Carica candamarcensis Hook.
Biol Chem Hoppe Seyler. 1993 Jul;374(7):501-6. doi: 10.1515/bchm3.1993.374.7-12.501.
9
The amino acid sequence of chymopapain from Carica papaya.番木瓜凝乳蛋白酶的氨基酸序列。
Biochem J. 1990 Feb 15;266(1):75-81. doi: 10.1042/bj2660075.
10
Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.糜蛋白酶M的结构：通过比较建模技术推导得出的晚期洗脱糜蛋白酶，以及通过pH依赖性催化动力学和与时间依赖性抑制剂反应确定的活性中心特征：半胱氨酸-25/组氨酸-159离子对对于糜蛋白酶M和木瓜蛋白酶的催化活性而言均不充分。
Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.
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Crystallization and preliminary X-ray diffraction studies of the precursor protein of a thermostable variant of papain.木瓜蛋白酶热稳定变体前体蛋白的结晶及初步X射线衍射研究
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):634-6. doi: 10.1107/S1744309111010888. Epub 2011 Apr 28.
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Preparation and characterization of a truncated caricain lacking 41 residues from the N-terminal.一种从N端缺失41个残基的截短型木瓜蛋白酶的制备与表征。
Protein J. 2005 May;24(4):243-51. doi: 10.1007/s10930-005-6720-5.
3
Purification and characterization of multiple forms of the pineapple-stem-derived cysteine proteinases ananain and comosain.菠萝茎来源的半胱氨酸蛋白酶菠萝蛋白酶和科莫萨因多种形式的纯化与表征
Biochem J. 1994 Aug 1;301 ( Pt 3)(Pt 3):727-35. doi: 10.1042/bj3010727.
4
Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.糜蛋白酶M的结构：通过比较建模技术推导得出的晚期洗脱糜蛋白酶，以及通过pH依赖性催化动力学和与时间依赖性抑制剂反应确定的活性中心特征：半胱氨酸-25/组氨酸-159离子对对于糜蛋白酶M和木瓜蛋白酶的催化活性而言均不充分。
Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.
5
Names and numbers of papaya proteinases.木瓜蛋白酶的名称和编号。
Biochem J. 1985 Jun 1;228(2):527. doi: 10.1042/bj2280527.
6
Molecular cloning of two cysteine proteinases from paw-paw (Carica papaya).从番木瓜（番木瓜属）中克隆出两种半胱氨酸蛋白酶的分子克隆。
Biochem J. 1986 Jul 1;237(1):105-10. doi: 10.1042/bj2370105.
7
Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives.木瓜凝乳蛋白酶A。通过共价色谱法进行纯化及研究，并利用双质子态反应探针动力学、稳态动力学以及某些二硫代酰基衍生物的共振拉曼光谱进行表征。
Biochem J. 1986 Jan 1;233(1):119-29. doi: 10.1042/bj2330119.
8
Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex.
Biochem J. 1989 Jul 15;261(2):469-76. doi: 10.1042/bj2610469.
9
Structure-function relationships in the cysteine proteinases actinidin, papain and papaya proteinase omega. Three-dimensional structure of papaya proteinase omega deduced by knowledge-based modelling and active-centre characteristics determined by two-hydronic-state reactivity probe kinetics and kinetics of catalysis.半胱氨酸蛋白酶肌动蛋白水解酶、木瓜蛋白酶和木瓜蛋白酶ω的结构-功能关系。通过基于知识的建模推导木瓜蛋白酶ω的三维结构，以及通过双水合态反应探针动力学和催化动力学确定其活性中心特征。
Biochem J. 1991 Nov 15;280 ( Pt 1)(Pt 1):79-92. doi: 10.1042/bj2800079.
本文引用的文献
1
Isolation of highly active papaya peptidases A and B from commercial chymopapain.
Biochim Biophys Acta. 1981 Apr 14;658(2):262-9. doi: 10.1016/0005-2744(81)90296-5.
2
Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (beta nuc.) for the reactions of thiolate anions with 2,2'-dipyridyl disulphide may be decreased by reagent protonation.有证据表明，木瓜凝乳蛋白酶A的活性中心与其他一些半胱氨酸蛋白酶的活性中心不同，并且硫醇盐阴离子与2,2'-二吡啶二硫化物反应的布仑斯惕系数（β nuc.）可能会因试剂质子化而降低。
Biochem J. 1980 Jul 1;189(1):189-29. doi: 10.1042/bj1890189.
3
Chymopapain. Chromatographic purification and immunological characterization.木瓜凝乳蛋白酶。色谱纯化及免疫学特性鉴定。
Biochem J. 1984 Oct 1;223(1):81-8. doi: 10.1042/bj2230081.
4
Problems of classification of papaya latex proteinases.番木瓜乳胶蛋白酶的分类问题。
Biochem J. 1984 Jul 15;221(2):555-6. doi: 10.1042/bj2210555.
5
'Chymopapain S' is chymopapain A.“木瓜凝乳蛋白酶S”即木瓜凝乳蛋白酶A。
Biochem J. 1984 Jul 15;221(2):553-4. doi: 10.1042/bj2210553.
6
A re-evaluation of the nomenclature of the cysteine proteinases of Carica papaya and a rational basis for their identification.番木瓜半胱氨酸蛋白酶命名的重新评估及其鉴定的合理依据。
Biochem J. 1983 Aug 1;213(2):559-60. doi: 10.1042/bj2130559.
7
Two-protonic-state electrophiles as probes of enzyme mechanisms.双质子态亲电试剂作为酶作用机制的探针
Methods Enzymol. 1982;87:427-69. doi: 10.1016/s0076-6879(82)87026-2.
8
Fractionation of proteolytic enzymes of dried papaya latex. Isolation and preliminary characterization of a new proteolytic enzyme.
C R Trav Lab Carlsberg. 1967;36(4):67-83.
9
Chymopapain. IV. The chromatographic fractionation of partially purified chymopapain and the characterization of crystalline chymopapain B.
Biochim Biophys Acta. 1967 Jul 11;139(2):405-17. doi: 10.1016/0005-2744(67)90044-7.
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