Chymopapain. IV. The chromatographic fractionation of partially purified chymopapain and the characterization of crystalline chymopapain B.

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作者信息

Kunimitsu D K, Yasunobu K T

出版信息

Biochim Biophys Acta. 1967 Jul 11;139(2):405-17. doi: 10.1016/0005-2744(67)90044-7.

DOI:10.1016/0005-2744(67)90044-7

PMID:6034682

Abstract

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相似文献

Chymopapain. IV. The chromatographic fractionation of partially purified chymopapain and the characterization of crystalline chymopapain B.

Biochim Biophys Acta. 1967 Jul 11;139(2):405-17. doi: 10.1016/0005-2744(67)90044-7.

Chymopapain A. Purification and investigation by covalent chromatography and characterization by two-protonic-state reactivity-probe kinetics, steady-state kinetics and resonance Raman spectroscopy of some dithioacyl derivatives.木瓜凝乳蛋白酶A。通过共价色谱法进行纯化及研究，并利用双质子态反应探针动力学、稳态动力学以及某些二硫代酰基衍生物的共振拉曼光谱进行表征。

Biochem J. 1986 Jan 1;233(1):119-29. doi: 10.1042/bj2330119.

Structure of chymopapain M the late-eluted chymopapain deduced by comparative modelling techniques and active-centre characteristics determined by pH-dependent kinetics of catalysis and reactions with time-dependent inhibitors: the Cys-25/His-159 ion-pair is insufficient for catalytic competence in both chymopapain M and papain.糜蛋白酶M的结构：通过比较建模技术推导得出的晚期洗脱糜蛋白酶，以及通过pH依赖性催化动力学和与时间依赖性抑制剂反应确定的活性中心特征：半胱氨酸-25/组氨酸-159离子对对于糜蛋白酶M和木瓜蛋白酶的催化活性而言均不充分。

Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.

Chymopapain. Chromatographic purification and immunological characterization.木瓜凝乳蛋白酶。色谱纯化及免疫学特性鉴定。

Biochem J. 1984 Oct 1;223(1):81-8. doi: 10.1042/bj2230081.

The thiol proteinases from the latex of Carica papaya L. IV. Proteolytic specificities of chymopapain and papaya proteinase omega determined by digestion of alpha-globin chains.

Biol Chem Hoppe Seyler. 1989 Aug;370(8):819-29. doi: 10.1515/bchm3.1989.370.2.819.

A necessary modification to the preparation of papain from any high-quality latex of Carica papaya and evidence for the structural integrity of the enzyme produced by traditional methods.对从任何优质番木瓜乳胶制备木瓜蛋白酶的必要改进以及传统方法生产的酶结构完整性的证据。

Biochem J. 1979 Feb 1;177(2):541-8. doi: 10.1042/bj1770541.

Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex.

Biochem J. 1989 Jul 15;261(2):469-76. doi: 10.1042/bj2610469.

Isolation of highly active papaya peptidases A and B from commercial chymopapain.

Biochim Biophys Acta. 1981 Apr 14;658(2):262-9. doi: 10.1016/0005-2744(81)90296-5.

'Chymopapain S' is chymopapain A.“木瓜凝乳蛋白酶S”即木瓜凝乳蛋白酶A。

Biochem J. 1984 Jul 15;221(2):553-4. doi: 10.1042/bj2210553.

ISOLATION AND PROPERTIES OF GLUTAMINE CYCLOTRANSFERASE OF DRIED PAPAYA LATEX.干番木瓜乳胶谷氨酰胺环转移酶的分离与性质

Biochim Biophys Acta. 1964 Nov 22;92:409-11. doi: 10.1016/0926-6569(64)90204-4.

引用本文的文献

Biochem J. 1994 Jun 15;300 ( Pt 3)(Pt 3):805-20. doi: 10.1042/bj3000805.

Evidence that the active centre of chymopapain A is different from the active centres of some other cysteine proteinases and that the Brønsted coefficient (beta nuc.) for the reactions of thiolate anions with 2,2'-dipyridyl disulphide may be decreased by reagent protonation.有证据表明，木瓜凝乳蛋白酶A的活性中心与其他一些半胱氨酸蛋白酶的活性中心不同，并且硫醇盐阴离子与2,2'-二吡啶二硫化物反应的布仑斯惕系数（β nuc.）可能会因试剂质子化而降低。

Biochem J. 1980 Jul 1;189(1):189-29. doi: 10.1042/bj1890189.

Chymopapain. Chromatographic purification and immunological characterization.木瓜凝乳蛋白酶。色谱纯化及免疫学特性鉴定。

Biochem J. 1984 Oct 1;223(1):81-8. doi: 10.1042/bj2230081.

A re-evaluation of the nomenclature of the cysteine proteinases of Carica papaya and a rational basis for their identification.番木瓜半胱氨酸蛋白酶命名的重新评估及其鉴定的合理依据。

Biochem J. 1983 Aug 1;213(2):559-60. doi: 10.1042/bj2130559.

Fresh non-fruit latex of Carica papaya contains papain, multiple forms of chymopapain A and papaya proteinase omega.新鲜的番木瓜非果实乳胶含有木瓜蛋白酶、多种形式的木瓜凝乳蛋白酶A和木瓜蛋白酶ω。

Biochem J. 1985 Jun 1;228(2):525-7. doi: 10.1042/bj2280525.

Biochem J. 1986 Jan 1;233(1):119-29. doi: 10.1042/bj2330119.

Biochem J. 1989 Jan 1;257(1):183-6. doi: 10.1042/bj2570183.

Affinity purification of the novel cysteine proteinase papaya proteinase IV, and papain from papaya latex.

Biochem J. 1989 Jul 15;261(2):469-76. doi: 10.1042/bj2610469.

The amino acid sequence of chymopapain from Carica papaya.番木瓜凝乳蛋白酶的氨基酸序列。

Biochem J. 1990 Feb 15;266(1):75-81. doi: 10.1042/bj2660075.

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