Shareef Abdullah Abubaker, Kheder Azad Hasan, Albarzinji Niaz, Karim Karim Jalal, Smail Shukur Wasman, Mahmood Abuabkr Abdalla, Amin Kawa
Department of Biology, College of Science, Salahaddin University-Erbil, Erbil, Iraq.
Physiotherapy Department Erbil Health and Medical Technical College, Erbil Polytechnic University, Erbil, Iraq.
Future Sci OA. 2025 Dec;11(1):2483628. doi: 10.1080/20565623.2025.2483628. Epub 2025 Mar 31.
This study examines the relationship between oxidative stress, anti-oxidative markers, and the Ala16Val SOD2 polymorphism in children with autism spectrum disorder (ASD) to better understand ASD severity and susceptibility.
The study included 80 children (40 with ASD and 40 controls) from Erbil City, Iraq.
Serum antioxidant markers, such as superoxide dismutase (SOD) and glutathione peroxidase (GPX), were significantly lower in ASD patients compared to controls (P = 0.036 and P < 0.001, respectively), while markers of oxidative damage, including malondialdehyde (MDA), nitric oxide (NO), and cytochrome C, were significantly elevated (P < 0.001). Regression analysis revealed reduced SOD and GPX activities were strongly associated with increased autism severity, as measured by the childhood autism rating scale (CARS), while elevated NO and cytochrome C levels also correlated positively with higher CARS scores. Although the Ala16Val SOD polymorphism was not significantly associated with ASD risk, logistic regression showed no connection between SOD genotypes and serum SOD levels.
These findings suggest oxidative stress and impaired antioxidant defense play critical roles in ASD severity.
本研究探讨氧化应激、抗氧化标志物与自闭症谱系障碍（ASD）儿童Ala16Val超氧化物歧化酶2（SOD2）基因多态性之间的关系，以更好地了解ASD的严重程度和易感性。
该研究纳入了来自伊拉克埃尔比勒市的80名儿童（40名ASD患儿和40名对照）。
与对照组相比，ASD患者血清中的抗氧化标志物，如超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）显著降低（分别为P = 0.036和P < 0.001），而氧化损伤标志物，包括丙二醛（MDA）、一氧化氮（NO）和细胞色素C则显著升高（P < 0.001）。回归分析显示，通过儿童自闭症评定量表（CARS）测量，SOD和GPX活性降低与自闭症严重程度增加密切相关，而NO和细胞色素C水平升高也与较高的CARS评分呈正相关。尽管Ala16Val SOD基因多态性与ASD风险无显著关联，但逻辑回归显示SOD基因型与血清SOD水平之间无关联。
这些发现表明氧化应激和抗氧化防御受损在ASD严重程度中起关键作用。
