Sumida C, Magdelenat H, Pasqualini J R
Breast Cancer Res Treat. 1985;5(2):165-9. doi: 10.1007/BF01805990.
Estrogen and progesterone receptor concentrations in cytosol and nucleus were measured in 21 primary breast cancer tumors. Twelve out of the 21 tumor samples were cytosol estrogen receptor positive, 8 of which contained only unoccupied estrogen binding sites in the cytosol, but 2 of the 9 'estrogen receptor negative' samples did contain cytosol binding sites already occupied by endogenous hormone. Four other 'estrogen receptor negative' tumors only showed nuclear binding sites. Only 3 of the 12 'estrogen receptor positive' tumors also contained progesterone receptors. All of these tumors also had estrogen receptor in the nucleus. However, three of the 17 'progesterone receptor negative' samples had progesterone receptor only in the nucleus. The present data indicate that 3 possible classes of 'false negative' tumors can be encountered: estrogen receptors occupied by endogenous hormone, tumors containing only nuclear estrogen receptors, and tumors having only nuclear progesterone receptors. Measurement of nuclear estrogen receptor together with the progesterone receptor provides further information on whether the estrogen receptor system is not only present but also functional, and should be of value in the prediction of hormone dependent breast cancer.
对21例原发性乳腺癌肿瘤的胞浆和细胞核中的雌激素受体和孕激素受体浓度进行了测定。21个肿瘤样本中有12个胞浆雌激素受体呈阳性，其中8个在胞浆中仅含有未被占据的雌激素结合位点，但9个“雌激素受体阴性”样本中有2个确实含有已被内源性激素占据的胞浆结合位点。另外4个“雌激素受体阴性”肿瘤仅显示细胞核结合位点。12个“雌激素受体阳性”肿瘤中只有3个也含有孕激素受体。所有这些肿瘤的细胞核中也都有雌激素受体。然而，17个“孕激素受体阴性”样本中有3个仅在细胞核中有孕激素受体。目前的数据表明，可能会遇到3种“假阴性”肿瘤：被内源性激素占据的雌激素受体、仅含有细胞核雌激素受体的肿瘤以及仅含有细胞核孕激素受体的肿瘤。测定细胞核雌激素受体和孕激素受体可提供关于雌激素受体系统是否不仅存在而且具有功能的进一步信息，这对于预测激素依赖性乳腺癌应具有价值。
