Jones R A
Biochim Biophys Acta. 1977 Jan 3;474(1):154-61. doi: 10.1016/0005-2787(77)90222-2.
In growing maize root tissue [14C]asparagine formation in inhibited and [14C]glutamine accumulation stimulated by treatment with cycloheximide or glutamine analogs such as azaserine. In contrast, puromycin enhances the accumulation of [14C]asparagine but not [14C]glutamine. Cycloheximide and puromycin alone inhibit protein synthesis. This is interpreted to mean that the alteration in amide metabolism following cycloheximide treatment is a direct result of the antibiotic acting as a glutamine analog. While cycloheximide is often the cytoplasmic protein synthesis inhibitor of choice due to its potency and rapid action, its assumed specificity of action of eukaryotes is doubtful.
在生长的玉米根组织中，用环己酰亚胺或谷氨酰胺类似物（如重氮丝氨酸）处理会抑制[14C]天冬酰胺的形成，并刺激[14C]谷氨酰胺的积累。相反，嘌呤霉素会增强[14C]天冬酰胺的积累，但不会增强[14C]谷氨酰胺的积累。单独使用环己酰亚胺和嘌呤霉素会抑制蛋白质合成。这被解释为意味着环己酰亚胺处理后酰胺代谢的改变是抗生素作为谷氨酰胺类似物起作用的直接结果。虽然环己酰亚胺由于其效力和快速作用，通常是首选的细胞质蛋白质合成抑制剂，但其假定的对真核生物的作用特异性值得怀疑。
