Wang Lin, Shao Li, Gao Yong-Chao, Liu Jing, Li Xu-Dong, Zhou Jie, Li Shuang-Feng, Song Yue-Lin, Liu Bo, Zhang Wei, Huang Wei-Hua
Department of Clinical Pharmacology, Xiangya Hospital, Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics and National Clinical Research Center for Geriatric Disorders, Central South University, Changsha, Hunan 410008, P. R. China.
Department of Pharmacognosy, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410128, P. R. China.
Am J Chin Med. 2025;53(2):567-596. doi: 10.1142/S0192415X25500223. Epub 2025 Mar 29.
Bile acid metabolism mediated by gut microbiota is significantly related to immunity regulation that plays an important role in the development and treatment of inflammatory bowel disease (IBD). Our previous study has demonstrated that saponins (PNS) alleviate colitis due to the regulation of T helper 17/Regulatory T cells (Th17/Treg) balance via gut microbiota. However, the effects and mechanism of PNS on colitis pertinent to bile acid metabolism mediated by gut microbiota remain elusive. This study aims to investigate the anti-colitis mechanism of PNS by regulating the Th17/Treg balance pertinent to gut microbiota-bile acid metabolism. Results showed that PNS significantly decreased the relative abundance of , , , and , and up-regulated the relative contents of conjugated bile acids, such as TCA and TCDCA. Fecal microbiota transplantation (FMT) showed that the gut microbiota remodeled by PNS had a regulatory effect on bile acid metabolism, and up-regulated the relative contents of TCA and TCDCA, which alleviated IBD and promoted Treg cell expression and . Taken together, PNS could reshape the profiling of gut microbiota to generate more TCA and TCDCA, which improve the balance of Th17/Treg to exert anti-IBD effects.
由肠道微生物群介导的胆汁酸代谢与免疫调节显著相关，而免疫调节在炎症性肠病（IBD）的发生发展及治疗中发挥着重要作用。我们之前的研究表明，人参皂苷（PNS）通过肠道微生物群调节辅助性T细胞17/调节性T细胞（Th17/Treg）平衡来减轻结肠炎。然而，PNS对由肠道微生物群介导的与胆汁酸代谢相关的结肠炎的影响及机制仍不清楚。本研究旨在通过调节与肠道微生物群 - 胆汁酸代谢相关的Th17/Treg平衡来探讨PNS的抗结肠炎机制。结果显示，PNS显著降低了[具体菌种名称1]、[具体菌种名称2]、[具体菌种名称3]和[具体菌种名称4]的相对丰度，并上调了结合胆汁酸如牛磺胆酸（TCA）和牛磺去氧胆酸（TCDCA）的相对含量。粪便微生物群移植（FMT）表明，由PNS重塑的肠道微生物群对胆汁酸代谢具有调节作用，并上调了TCA和TCDCA的相对含量，从而减轻了IBD并促进了Treg细胞表达[具体基因名称1]和[具体基因名称2]。综上所述，PNS可重塑肠道微生物群谱以产生更多的TCA和TCDCA，从而改善Th17/Treg平衡以发挥抗IBD作用。 （注：原文中部分具体菌种名称和基因名称未给出完整信息，翻译时用[具体菌种名称X]和[具体基因名称X]表示）
