Huang Zichen, Wang Jiannan, He Xin, Zhang Mengyang, Ren Xingyue, Yu Wenya, Yao Sheng, Ji Kongshu
State Key Laboratory of Tree Genetics and Breeding, Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, China.
Key Laboratory of Forestry Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, Nanjing, China.
Front Microbiol. 2025 Mar 17;16:1537173. doi: 10.3389/fmicb.2025.1537173. eCollection 2025.
In subtropical China, is a timber tree species which have a great potential for carbon sequestration. However, few studies have investigated how varying levels of carbon storage in provenances affect the soil microbial functional potential related to nutrient cycling within the rhizosphere.
In this investigation, metagenomic sequencing was employed to explore the differences in carbon and nitrogen cycling capabilities among rhizosphere microbial communities within provenances, categorized by high, medium, and low levels of carbon storage.
Our findings revealed a significant increase in the relative abundance of and by 23 and 61%, respectively, whereas significantly decreased by 8% in the rhizosphere of provenances with high carbon storage compared with those with low carbon storage. The variability in carbon storage among provenances was linked to marked disparities in the presence of key genes essential for carbon and nitrogen cycling within their rhizosphere soils.
Notably, in provenances characterized by high carbon storage, the rhizosphere presented a significantly elevated presence of genes associated with carbon decomposition, carbon assimilation, methane generation, and denitrification, in stark contrast to provenances with medium and low carbon storage. Furthermore, provenances with high carbon storage rates presented increased transformation and availability of soil carbon and nitrogen, along with increased potential for ecological restoration. Moreover, the rhizosphere soil nitrification of provenances with low carbon storage surpassed that of other provenances, leading to increased available nitrogen content and elevated nitrate leaching risk. In the rhizosphere, critical soil factors, including soil organic carbon (SOC), total nitrogen (TN), pH, and nitrate nitrogen (NO -N) content, significantly shape the functionality of genes associated with carbon and nitrogen cycling. In conclusion, our study lays a scientific foundation for establishing plantations and identifying provenances with superior ecological value and potential.
在中国亚热带地区，[树种名称]是一种具有巨大碳固存潜力的用材树种。然而，很少有研究调查该树种不同种源的碳储存水平如何影响根际内与养分循环相关的土壤微生物功能潜力。
在本研究中，采用宏基因组测序来探究按高、中、低水平碳储存分类的[树种名称]种源根际微生物群落之间碳和氮循环能力的差异。
我们的研究结果显示，与低碳储存种源相比，高碳储存种源的根际中[特定微生物类群1]和[特定微生物类群2]的相对丰度分别显著增加了23%和61%，而[特定微生物类群3]显著减少了8%。[树种名称]种源间碳储存的差异与它们根际土壤中碳和氮循环关键基因的存在显著差异有关。
值得注意的是，在高碳储存的[树种名称]种源中，根际呈现出与碳分解、碳同化、甲烷生成和反硝化相关基因的显著增加，这与中、低碳储存种源形成鲜明对比。此外，高碳储存率的[树种名称]种源表现出土壤碳和氮的转化和有效性增加，以及生态恢复潜力增加。而且，低碳储存种源的根际土壤硝化作用超过其他种源，导致有效氮含量增加和硝酸盐淋溶风险升高。在[树种名称]根际，关键土壤因子，包括土壤有机碳（SOC）、总氮（TN）、pH和硝态氮（NO₃-N）含量，显著影响与碳和氮循环相关基因的功能。总之，我们的研究为建立[树种名称]人工林以及识别具有卓越生态价值和潜力的[树种名称]种源奠定了科学基础。
