Vörösházi Júlia, Mackei Máté, Sebők Csilla, Tráj Patrik, Márton Rege Anna, Neogrády Zsuzsanna, Mátis Gábor
Division of Biochemistry, Department of Physiology and Biochemistry, University of Veterinary Medicine, Budapest, 1078, Hungary.
National Laboratory of Infectious Animal Diseases, Antimicrobial Resistance, Veterinary Public Health and Food Chain Safety, University of Veterinary Medicine, Budapest, 1078, Hungary.
Sci Rep. 2025 Apr 1;15(1):11180. doi: 10.1038/s41598-025-95868-0.
Deoxynivalenol (DON) is a trichothecene mycotoxin often contaminating grains used in poultry feed production and causing several adverse effects in farm animals. Therefore, it is important to investigate compounds that can be potential candidates to mitigate these effects, such as baicalin. The effects of DON and baicalin were investigated in chicken-derived 3D hepatic cell cultures, and cell viability, LDH activity, oxidative parameters (NRF-2, 8-OHdG) and inflammatory parameters (IL-6, IL-8, IFN-γ) were monitored for 24 and 48 h. Our results suggest that DON reduced cellular metabolic activity but did not prove to be cytotoxic, and baicalin was able to attenuate this adverse effect. The change in extracellular LDH activity suggests that after 48 h the cells have already started to respond to the adverse effects of the toxin and protective mechanisms were induced. Based on the measured oxidative parameters, baicalin showed antioxidant activity, but after longer exposure, our results indicate a prooxidant effect. Baicalin also had an anti-inflammatory effect based on the amount of IL-6 and IL-8, while DON exerted a dose-and time-dependent pleiotropic activity. These results suggest that DON may have an impact on cellular inflammation and oxidative homeostasis, and that baicalin could be able to alleviate these adverse effects.
脱氧雪腐镰刀菌烯醇（DON）是一种单端孢霉烯族真菌毒素，常污染家禽饲料生产中使用的谷物，并对农场动物造成多种不良影响。因此，研究能够减轻这些影响的潜在化合物非常重要，比如黄芩苷。我们在鸡源三维肝细胞培养物中研究了DON和黄芩苷的作用，并在24小时和48小时内监测了细胞活力、乳酸脱氢酶（LDH）活性、氧化参数（NRF-2、8-羟基脱氧鸟苷）和炎症参数（白细胞介素-6、白细胞介素-8、干扰素-γ）。我们的结果表明，DON降低了细胞代谢活性，但未被证明具有细胞毒性，而黄芩苷能够减轻这种不良影响。细胞外LDH活性的变化表明，48小时后细胞已经开始对毒素的不良影响做出反应，并诱导了保护机制。基于所测得的氧化参数，黄芩苷显示出抗氧化活性，但长时间暴露后，我们的结果表明其具有促氧化作用。基于白细胞介素-6和白细胞介素-8的含量，黄芩苷也具有抗炎作用，而DON则表现出剂量和时间依赖性的多效活性。这些结果表明，DON可能对细胞炎症和氧化稳态产生影响，而黄芩苷能够减轻这些不良影响。
