Negrescu Andreea Mariana, Nistorescu Simona, Bonciu Anca, Rusen Laurentiu, Dumitrescu Nicoleta, Urzica Iuliana, Moldovan Antoniu, Hoffmann Patrick, Pircalaboiu Gratiela Gradisteanu, Cimpean Anisoara, Dinca Valentina
Faculty of Biology, University of Bucharest Splaiul Independenţei 91-95 050095 Bucharest Romania
Research Institute of the University of Bucharest (ICUB), University of Bucharest 050657 Bucharest Romania.
RSC Adv. 2025 Apr 1;15(13):9952-9967. doi: 10.1039/d5ra00063g. eCollection 2025 Mar 28.
Even today, the reduction of complications following breast implant surgery together with the enhancement of implant integration and performance through the modulation of the foreign body response (FBR), remains a fundamental challenge in the field of plastic surgery. Therefore, tailoring the material's physical characteristics to modulate FBR can represent an effective approach in implantology. While polydimethylsiloxane (PDMS) patterning on 2D substrates is a relatively established and available procedure, micropatterning multiscaled biointerfaces on a controlled large area has been more challenging. Therefore, in the present work, a specific designed honeycomb-like well biointerface was designed and obtained by replication in PDMS at large scale and its effectiveness towards creating a pro-healing environment was investigated. The grayscale masks assisted laser-based 3D texturing method was used for creating the required moulds in Polycarbonate for large area replication. By comparison to the smooth substrate, the honeycomb topography altered the fibroblasts' behaviour in terms of adhesion and morphology and reduced the macrophages' inflammatory response. Additionally, the microstructured surface effectively inhibited macrophage fusion, significantly limiting the colonization of both Gram-positive and Gram-negative microbial strains on the tested surfaces. Overall, this study introduces an innovative approach to mitigate the FBR to silicone, achieved through the creation of a honeycomb-inspired topography for prosthetic interfaces.
即使在今天，减少乳房植入手术的并发症以及通过调节异物反应（FBR）来增强植入物的整合和性能，仍然是整形外科领域的一项基本挑战。因此，调整材料的物理特性以调节FBR可能是植入学中的一种有效方法。虽然在二维基板上进行聚二甲基硅氧烷（PDMS）图案化是一种相对成熟且可行的程序，但在可控大面积上进行微图案化多尺度生物界面则更具挑战性。因此，在本研究中，设计并通过大规模PDMS复制获得了一种特定设计的蜂窝状微孔生物界面，并研究了其在创建促进愈合环境方面的有效性。采用灰度掩模辅助的基于激光的3D纹理化方法在聚碳酸酯中制作大面积复制所需的模具。与光滑基板相比，蜂窝状拓扑结构改变了成纤维细胞的粘附和形态行为，并降低了巨噬细胞的炎症反应。此外，微结构化表面有效抑制了巨噬细胞融合，显著限制了革兰氏阳性和革兰氏阴性微生物菌株在测试表面的定植。总体而言，本研究介绍了一种创新方法，通过为假体界面创建受蜂窝启发的拓扑结构来减轻对硅酮的FBR。
