Calta Evelyn, de Groot Anton, van Oers Emma M, Ipenburg Norbertus A, Rustemeyer Thomas
Kurt Kitzing GmbH, Wallerstein, Germany.
Dermato-Allergology and Occupational Dermatology, Amsterdam University Medical Centers, Amsterdam, the Netherlands.
Contact Dermatitis. 2025 Jul;93(1):56-60. doi: 10.1111/cod.14796. Epub 2025 Apr 2.
In Amsterdam, in 2024, patch testing with Brazilian propolis yielded high rates of positive reactions (> 20%), whereas reactivity to Chinese propolis was significantly lower (3.5%). Differences in the composition were suggested as a possible explanation.
Analyses were performed using gas chromatography-mass spectrometry/flame ionisation detection (GC-MS/FID) of the volatile components obtained by headspace SPME (solid phase microextraction).
A strong difference between the composition of the Brazilian propolis sample and both samples of Chinese propolis was found. Major ingredients in Brazilian propolis were hydrocinnamic acid (16.9%), (E)-nerolidol (7.41%), spathulenol (5.45%) and junenol (4.01%). Major ingredients in Chinese propolis were (E)-cinnamyl alcohol (8.08% and 24.96%), 2-phenethyl alcohol (8.93% and 11.25%), α-curcumene (8.77% and 8.81%) and guaiol (5.96% and 5.72%).
The volatile fractions of Brazilian propolis and Chinese propolis used for patch testing have very different compositions. Whether this causes or contributes to the differences in patch test reactivity has to be investigated further.
2024年在阿姆斯特丹，对巴西蜂胶进行斑贴试验时阳性反应率很高（>20%），而对中国蜂胶的反应性则显著较低（3.5%）。有人认为成分差异可能是一个解释。
使用气相色谱-质谱联用/火焰离子化检测（GC-MS/FID）对头空间固相微萃取（SPME）获得的挥发性成分进行分析。
发现巴西蜂胶样品与两种中国蜂胶样品的成分存在很大差异。巴西蜂胶的主要成分是氢化肉桂酸（16.9%）、（E）-橙花叔醇（7.41%）、匙叶桉油烯醇（5.45%）和紫穗槐醇（4.01%）。中国蜂胶的主要成分是（E）-肉桂醇（8.08%和24.96%）、2-苯乙醇（8.93%和11.25%）、α-姜黄烯（8.77%和8.81%）和愈创木醇（5.96%和5.72%）。
用于斑贴试验的巴西蜂胶和中国蜂胶的挥发性成分组成差异很大。这是否导致或促成了斑贴试验反应性的差异还有待进一步研究。
