de Groot Anton, Calta Evelyn, Ipenburg Norbertus A, van Oers Emma M, Rustemeyer Thomas
Dermato-Allergology and Occupational Dermatology, Amsterdam University Medical Centers, Amsterdam, the Netherlands.
Kurt Kitzing GmbH, Wallerstein, Germany.
Contact Dermatitis. 2025 Aug;93(2):108-113. doi: 10.1111/cod.14807. Epub 2025 May 4.
In patch testing, co-reactivity between Myroxylon pereirae resin, colophonium and propolis is well recognised. One of the possible explanations is that these materials have common allergenic ingredients.
To identify the main ingredients in M. pereirae resin and colophonium samples used in the preparation of commercial patch test allergens and to compare their compositions with each other as well as with propolis.
Analyses were performed on M. pereirae resin and colophonium samples using gas chromatography-mass spectrometry/flame ionisation detection of the volatile components obtained by headspace SPME (solid phase microextraction).
The main ingredients in M. pereirae resin were benzyl benzoate, (E)-nerolidol, benzoic acid, benzyl alcohol, (E)-cinnamic acid, vanillin and (E)-benzyl cinnamate. In colophonium, longifolene, caryophyllene oxide, acetone + formic acid, α-terpineol and δ-cadinene + calamenene were the major constituents.
The major ingredients of the volatile fractions of M. pereirae resin and colophonium are quite different; common haptens in volatile ingredients cannot readily explain co-reactivity. M. pereirae resin has cinnamic acid- and benzoic acid derivatives in common with propolis and in addition (E)-nerolidol and vanillin with Brazilian propolis and benzyl alcohol with Chinese propolis. Colophonium shares various ingredients with Brazilian propolis but few with the Chinese variety.
在斑贴试验中，秘鲁香脂、松香和蜂胶之间的交叉反应性已得到充分认识。一种可能的解释是这些物质具有共同的致敏成分。
鉴定用于制备商业斑贴试验变应原的秘鲁香脂树脂和松香样品中的主要成分，并将它们的成分相互比较以及与蜂胶进行比较。
采用顶空固相微萃取（SPME）获得挥发性成分，然后用气相色谱 - 质谱联用/火焰离子化检测法对秘鲁香脂树脂和松香样品进行分析。
秘鲁香脂树脂中的主要成分是苯甲酸苄酯、（E）-橙花叔醇、苯甲酸、苯甲醇、（E）-肉桂酸、香草醛和（E）-肉桂酸苄酯。在松香中，长叶烯、氧化石竹烯、丙酮 + 甲酸、α-松油醇和δ-杜松烯 + 白菖烯是主要成分。
秘鲁香脂树脂和松香挥发性成分的主要成分有很大差异；挥发性成分中的常见半抗原不能轻易解释交叉反应性。秘鲁香脂树脂与蜂胶有共同的肉桂酸和苯甲酸衍生物，此外，（E）-橙花叔醇和香草醛与巴西蜂胶相同，苯甲醇与中国蜂胶相同。松香与巴西蜂胶有多种共同成分，但与中国蜂胶的共同成分很少。
