Christenson R H, Dent G A, Tuszynski A
Clin Chem. 1985 Aug;31(8):1358-60.
For 154 subjects, we verified that vitamin B12 and folate status was normal, using as criteria the average polymorphonuclear lobe count, mean corpuscular volume, and hemoglobin concentration. We then used blood from these subjects to compare values obtained with two radioassay kits, each designed for simultaneous vitamin B12 and folate determination. Although regression analysis showed reasonable correlation between the folate (r = 0.87) and vitamin B12 (r = 0.94) kits, we observed significant differences in the overall mean values for vitamin B12 (p less than 0.01) and folate (p less than 0.001) as measured with the kits in this population. Radioassay standard-curve data for the folate assays were similar, but these data indicated greater sensitivity at low concentrations for one vitamin B12 kit than the other. Using reference intervals recommended in the kit inserts, we found that the vitamin B12 status for 9% of these subjects would have been misclassified by one kit, 2% by the other.
对于154名受试者，我们以多形核白细胞叶平均数、平均红细胞体积和血红蛋白浓度为标准，验证了维生素B12和叶酸状态正常。然后我们使用这些受试者的血液来比较用两种放射免疫分析试剂盒获得的值，每种试剂盒都设计用于同时测定维生素B12和叶酸。尽管回归分析显示叶酸试剂盒（r = 0.87）和维生素B12试剂盒（r = 0.94）之间存在合理的相关性，但我们观察到在该人群中用试剂盒测得的维生素B12（p < 0.01）和叶酸（p < 0.001）的总体平均值存在显著差异。叶酸检测的放射免疫分析标准曲线数据相似，但这些数据表明，一种维生素B12试剂盒在低浓度下比另一种更具敏感性。使用试剂盒说明书中推荐的参考区间，我们发现这些受试者中有9%的维生素B12状态会被一种试剂盒错误分类，2%会被另一种试剂盒错误分类。
