• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

一种具有活性氧响应释放模式的工程强化细胞外囊泡整合水凝胶可减轻脊髓损伤。

An engineering-reinforced extracellular vesicle-integrated hydrogel with an ROS-responsive release pattern mitigates spinal cord injury.

作者信息

Cao Jian, Zhang Xunqi, Guo Jing, Wu Jiahe, Lin Lingmin, Lin Xurong, Mu Jiafu, Huang Tianchen, Zhu Manning, Ma Lan, Zhou Weihang, Jiang Xinchi, Wang Xuhua, Feng Shiqing, Gu Zhen, Gao Jian-Qing

机构信息

State Key Laboratory of Advanced Drug Delivery and Release Systems, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.

Department of Rehabilitation Medicine of First Affiliated Hospital and School of Brain Science and Brain Medicine, Zhejiang University School of Medicine, Hangzhou 310003, China.

出版信息

Sci Adv. 2025 Apr 4;11(14):eads3398. doi: 10.1126/sciadv.ads3398. Epub 2025 Apr 2.

DOI:10.1126/sciadv.ads3398
PMID:40173229
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11963969/
Abstract

The local delivery of mesenchymal stem cell-derived extracellular vesicles (EVs) via hydrogel has emerged as an effective approach for spinal cord injury (SCI) treatment. However, achieving on-demand release of EVs from hydrogel to address dynamically changing pathology remains challenging. Here, we used a series of engineering methods to further enhance EVs' efficacy and optimize their release pattern from hydrogel. Specifically, the pro-angiogenic, neurotrophic, and anti-inflammatory effects of EVs were reinforced through three-dimensional culture and dexamethasone (Dxm) encapsulation. Then, the prepared Dxm-loaded 3EVs (3EVs-Dxm) were membrane modified with ortho-dihydroxy groups (-2OH) and formed an EV-integrated hydrogel (3EVs-Dxm-Gel) via the cross-link with phenylboronic acid-modified hyaluronic acid and tannic acid. The phenylboronic acid ester in 3EVs-Dxm-Gel enabled effective immobilization and reactive oxygen species-responsive release of EVs. Topical injection of 3EVs-Dxm-Gel in SCI rats notably mitigated injury severity and promoted functional recovery, which may offer opportunities for EV-based therapeutics in central nervous system injury.

摘要

通过水凝胶局部递送间充质干细胞衍生的细胞外囊泡（EVs）已成为治疗脊髓损伤（SCI）的一种有效方法。然而，实现EVs从水凝胶中的按需释放以应对动态变化的病理状况仍然具有挑战性。在此，我们使用了一系列工程方法来进一步提高EVs的疗效，并优化其从水凝胶中的释放模式。具体而言，通过三维培养和地塞米松（Dxm）包封增强了EVs的促血管生成、神经营养和抗炎作用。然后，将制备的载有Dxm的3EVs（3EVs-Dxm）用邻二羟基（-2OH）进行膜修饰，并通过与苯基硼酸修饰的透明质酸和单宁酸交联形成EV整合水凝胶（3EVs-Dxm-Gel）。3EVs-Dxm-Gel中的苯基硼酸酯能够有效固定EVs并实现活性氧响应性释放。在SCI大鼠中局部注射3EVs-Dxm-Gel可显著减轻损伤严重程度并促进功能恢复，这可能为中枢神经系统损伤的基于EVs的治疗提供机会。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/5bc858037ef9/sciadv.ads3398-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/82f845561cec/sciadv.ads3398-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/3daa03b794aa/sciadv.ads3398-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/138a7b260e0b/sciadv.ads3398-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/cd05b1036fce/sciadv.ads3398-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/a7b310061f5d/sciadv.ads3398-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/5bc858037ef9/sciadv.ads3398-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/82f845561cec/sciadv.ads3398-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/3daa03b794aa/sciadv.ads3398-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/138a7b260e0b/sciadv.ads3398-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/cd05b1036fce/sciadv.ads3398-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/a7b310061f5d/sciadv.ads3398-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3fd6/11963969/5bc858037ef9/sciadv.ads3398-f6.jpg

相似文献

1
An engineering-reinforced extracellular vesicle-integrated hydrogel with an ROS-responsive release pattern mitigates spinal cord injury.一种具有活性氧响应释放模式的工程强化细胞外囊泡整合水凝胶可减轻脊髓损伤。
Sci Adv. 2025 Apr 4;11(14):eads3398. doi: 10.1126/sciadv.ads3398. Epub 2025 Apr 2.
2
Injectable Hydrogel Loaded with CDs and FTY720 Combined with Neural Stem Cells for the Treatment of Spinal Cord Injury.载有 CDs 和 FTY720 的可注射水凝胶与神经干细胞联合用于治疗脊髓损伤。
Int J Nanomedicine. 2024 May 8;19:4081-4101. doi: 10.2147/IJN.S448962. eCollection 2024.
3
IL-4-primed human umbilical cord mesenchymal stem cells-derived extracellular vesicles facilitate recovery in spinal cord injury via the miR-21-5p/PDCD4-mediated shifting of macrophage M1/M2 polarization.白细胞介素-4预处理的人脐带间充质干细胞衍生的细胞外囊泡通过miR-21-5p/程序性细胞死亡蛋白4介导的巨噬细胞M1/M2极化转变促进脊髓损伤的恢复。
Life Sci. 2025 Mar 1;364:123441. doi: 10.1016/j.lfs.2025.123441. Epub 2025 Feb 3.
4
Local delivery of mesenchymal stem cell-extruded nanovesicles through a bio-responsive scaffold for acute spinal cord injury treatment.通过生物响应性支架局部递送间充质干细胞分泌的纳米囊泡用于急性脊髓损伤治疗。
Int J Pharm. 2025 Feb 25;671:125222. doi: 10.1016/j.ijpharm.2025.125222. Epub 2025 Jan 13.
5
Hypoxic-preconditioned mesenchymal stem cell-derived small extracellular vesicles promote the recovery of spinal cord injury by affecting the phenotype of astrocytes through the miR-21/JAK2/STAT3 pathway.缺氧预处理间充质干细胞来源的小细胞外囊泡通过 miR-21/JAK2/STAT3 通路影响星形胶质细胞表型促进脊髓损伤恢复。
CNS Neurosci Ther. 2024 Mar;30(3):e14428. doi: 10.1111/cns.14428. Epub 2023 Aug 29.
6
Bone mesenchymal stem cell-derived extracellular vesicles deliver microRNA-23b to alleviate spinal cord injury by targeting toll-like receptor TLR4 and inhibiting NF-κB pathway activation.骨髓间充质干细胞来源的细胞外囊泡通过靶向 Toll 样受体 TLR4 并抑制 NF-κB 通路激活来递送 microRNA-23b，从而缓解脊髓损伤。
Bioengineered. 2021 Dec;12(1):8157-8172. doi: 10.1080/21655979.2021.1977562.
7
A sequential stimuli-responsive hydrogel promotes structural and functional recovery of severe spinal cord injury.一种顺序刺激响应性水凝胶促进严重脊髓损伤的结构和功能恢复。
Biomaterials. 2025 May;316:122995. doi: 10.1016/j.biomaterials.2024.122995. Epub 2024 Dec 5.
8
Extracellular vesicles derived from CD73 modified human umbilical cord mesenchymal stem cells ameliorate inflammation after spinal cord injury.CD73 修饰的人脐带间充质干细胞来源的细胞外囊泡减轻脊髓损伤后的炎症反应。
J Nanobiotechnology. 2021 Sep 8;19(1):274. doi: 10.1186/s12951-021-01022-z.
9
A reactive oxygen species-responsive hydrogel loaded with Apelin-13 promotes the repair of spinal cord injury by regulating macrophage M1/M2 polarization and neuroinflammation.负载Apelin-13的活性氧响应水凝胶通过调节巨噬细胞M1/M2极化和神经炎症促进脊髓损伤修复。
J Nanobiotechnology. 2025 Jan 10;23(1):12. doi: 10.1186/s12951-024-02978-4.
10
Extracellular vesicle-derived miR-511-3p from hypoxia preconditioned adipose mesenchymal stem cells ameliorates spinal cord injury through the TRAF6/S1P axis.缺氧预处理脂肪间充质干细胞来源的细胞外囊泡中的 miR-511-3p 通过 TRAF6/S1P 轴改善脊髓损伤。
Brain Res Bull. 2022 Mar;180:73-85. doi: 10.1016/j.brainresbull.2021.12.015. Epub 2021 Dec 31.

引用本文的文献

1
Emerging regenerative strategies for spinal cord injury: exosome-derived mechanisms and therapeutic insights.脊髓损伤的新兴再生策略：外泌体衍生机制与治疗见解。
Front Neurosci. 2025 Aug 25;19:1652196. doi: 10.3389/fnins.2025.1652196. eCollection 2025.
2
Genetically engineered MSC-derived hybrid cellular vesicles for ROS-scavenging and mitochondrial homeostasis in hepatic ischemia-reperfusion injury.用于肝缺血再灌注损伤中清除活性氧和维持线粒体稳态的基因工程间充质干细胞来源的杂交细胞囊泡
Mater Today Bio. 2025 Aug 19;34:102215. doi: 10.1016/j.mtbio.2025.102215. eCollection 2025 Oct.
3
Extracellular Vesicles as Emerging Therapeutic Strategies in Spinal Cord Injury: Ready to Go.

本文引用的文献

1
ROS-responsive hydrogels: from design and additive manufacturing to biomedical applications.ROS响应性水凝胶：从设计、增材制造到生物医学应用
Mater Horiz. 2024 Aug 12;11(16):3721-3746. doi: 10.1039/d4mh00289j.
2
Encapsulation and assessment of therapeutic cargo in engineered exosomes: a systematic review.工程化外泌体中治疗性货物的包封和评估：系统评价。
J Nanobiotechnology. 2024 Jan 3;22(1):18. doi: 10.1186/s12951-023-02259-6.
3
Initial IL-10 production dominates the therapy of mesenchymal stem cell scaffold in spinal cord injury.
细胞外囊泡作为脊髓损伤新兴的治疗策略：蓄势待发。
Biomedicines. 2025 May 21;13(5):1262. doi: 10.3390/biomedicines13051262.
初始的 IL-10 产生主导了间充质干细胞支架在脊髓损伤中的治疗作用。
Theranostics. 2024 Jan 1;14(2):879-891. doi: 10.7150/thno.87843. eCollection 2024.
4
Schwann Cell-Derived Exosomes and Methylprednisolone Composite Patch for Spinal Cord Injury Repair.雪旺细胞衍生外泌体与甲泼尼龙复合贴剂修复脊髓损伤。
ACS Nano. 2023 Nov 28;17(22):22928-22943. doi: 10.1021/acsnano.3c08046. Epub 2023 Nov 10.
5
Global, regional, and national burden of spinal cord injury, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019.全球、区域和国家脊髓损伤负担，1990-2019 年：2019 年全球疾病负担研究的系统分析。
Lancet Neurol. 2023 Nov;22(11):1026-1047. doi: 10.1016/S1474-4422(23)00287-9.
6
Global, regional and national burden of traumatic brain injury and spinal cord injury, 1990-2019: a systematic analysis for the Global Burden of Disease Study 2019.全球、区域和国家创伤性脑损伤和脊髓损伤负担，1990-2019 年：2019 年全球疾病负担研究的系统分析。
BMJ Open. 2023 Oct 6;13(10):e075049. doi: 10.1136/bmjopen-2023-075049.
7
Engineering strategies and optimized delivery of exosomes for theranostic application in nerve tissue.工程策略和外泌体的优化递送用于神经组织的治疗应用。
Theranostics. 2023 Jul 24;13(12):4266-4286. doi: 10.7150/thno.84971. eCollection 2023.
8
Stem cell-derived exosome versus stem cell therapy.干细胞衍生外泌体与干细胞疗法
Nat Rev Bioeng. 2023 Apr 12:1-2. doi: 10.1038/s44222-023-00064-2.
9
Spinal cord injury: molecular mechanisms and therapeutic interventions.脊髓损伤：分子机制与治疗干预。
Signal Transduct Target Ther. 2023 Jun 26;8(1):245. doi: 10.1038/s41392-023-01477-6.
10
Regulation of axonal regeneration after mammalian spinal cord injury.哺乳动物脊髓损伤后的轴突再生调控。
Nat Rev Mol Cell Biol. 2023 Jun;24(6):396-413. doi: 10.1038/s41580-022-00562-y. Epub 2023 Jan 5.